再生水灌溉对玉米和大豆抗氧化酶系统的影响

Influence of Irrigation with Reclaimed Water on Maize and Soybean Antioxdase System

再生水应用于农田灌溉是缓解农业用水紧张和解决污水环境污染的重要措施。作物抗氧化酶系统变化是其对外界环境因素变化响应的较早预警指标。本研究采用盆栽试验方法,以清水和原污水灌溉为对照,比较了二级再生水和三级再生水灌溉对玉米(Zeamays L.)和大豆(Phaseolus vulgaris L.)不同生长阶段叶片中超氧化歧化酶(SOD)、过氧化物酶(POD)和丙二醛(MDA)的影响。结果表明,与清水对照相比,二级再生水灌溉对玉米苗期抗氧化酶系统有一定影响,对玉米后期抗氧化酶系统的影响减小;三级再生水灌溉对玉米抗氧化酶系统影响不显著。再生水灌溉对大豆苗期和收获期抗氧化酶系统影响较小,但对其花期生长产生较强的氧化胁迫。这些结果极大丰富了再生水农田安全灌溉应用基础。

Irrigation with reclaimed water is an important way to deal with water shortage of agricultural production and solve the problem of sewage pollution. The reaction of crop antioxidant enzyme system is an early-warning indicator responding to changing of environmental factors. Using fresh water and raw wastewater as controls, the pot experiment was carried out to study the reaction of peroxides（POD）, superoxi dedismutase （SOD） activities and malondialdehyde（MDA） in leaf of maize （Zea mays L.） and soybean （Phaseolus vulgaris L.） irrigated with second grade and third grade reclaimed water. The results showed that the second grade reclaimed water had some negative influence on antioxdase system of maize in seedling stage, and the system decreased in later stage of maize; the third grade reclaimed water had no significant effect on antioxdase system of maize. Irrigation with reclaimed water had little influence on antioxdase system of soybean in seeding and reap- ing stage, but resulted in some oxidization stress in blossom stage. These results are very useful for safe irrigation with reclaimed water in agrlcultural production.