摘要
从石油污染土壤中分离得到1株降解石油烃类污染物的He4菌株.该菌株能够以正十六烷、苯、萘、蒽、菲和芘作为唯一的碳源生长.经过对其形态特征、生理生化、以及16SrRNA基因序列分析,该菌株初步鉴定为Gordoniasp..通过分析其16SrRNA基因序列,设计引物并构建了竞争性模板.通过竞争性定量PCR(quantitative competitive-PCR)分析了该菌株在含有菲的污染土壤中数量的变化.结果表明,部分菌株He4在土壤中转变为不可培养状态,采用传统的稀释涂布菌落计数法(CFU)无法对其进行定量,而通过QC-PCR能够较准确地测定土壤中微生物的动态变化.
A bacterial swain, He4, capable of degrading n-hexadecane and other polycyclic aromatic compounds was isolated from petroleum polluted soil. This strain was identified as Gordonia sp. He4 according to its morphology, physiological, biochemical properties and the analysis of its 16S rRNA gene sequence. Based on its 16S rRNA gene sequence, specific primers were designed and a competitor template was amplified by PCR. The dynamics of strain He4 in phenanthrene polluted soil was analyzed by colony forming unit (CFU) method and QC-PCR method. The results showed that partial of He4 become non-cuhurable and un-detectable by CFU method. But by using QC-PCR, the population density of strain He4 could be measured accurately.
出处
《环境科学》
EI
CAS
CSCD
北大核心
2007年第3期617-622,共6页
Environmental Science
基金
国家高技术研究发展计划(863)项目(2002AA601150)
中国科学院百人计划项目(BRJH-200)
关键词
多环芳烃
生物降解
生物修复
QC—PCR
polycyclic aromatic compounds
biodegradation
bioremediation
quantitative competitive PCR(QC-PCR)