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HPLC/MS^n法鉴定环境激素邻苯二甲酸丁基苄酯在小鼠尿中的代谢产物 被引量：5

Identification of Metabolites of Environmental Hormone Butylbenzyl Phthalate in Mice Urine by Liquid Chromatography/Ion Trap Mass Spectrometry
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摘要 应用HPLC-MSn方法,研究了邻苯二甲酸丁基苄酯在小鼠尿液中的代谢产物,并对尿中推测的代谢物和标准品分别进行HPLC-UV和二级全扫描质谱(MS2)分析,结果在尿中发现了6个代谢产物,分别为邻苯二甲酸、马脲酸、邻苯二甲酸单丁酯、邻苯二甲酸苄酯、邻苯二甲酸单丁酯葡萄糖醛酸结合物及邻苯二甲酸苄酯葡萄糖醛酸结合物.推断环境激素邻苯二甲酸丁基苄酯在小鼠体内的代谢途径为原形首先水解生成邻苯二甲酸单丁酯、邻苯二甲酸苄酯、邻苯二甲酸,部分邻苯二甲酸发生脱羧反应生成苯甲酸,苯甲酸再与内源性物质甘氨酸结合成马脲酸,邻苯二甲酸单丁酯、邻苯二甲酸苄酯和内源性β-D-葡萄糖醛酸结合,生成水溶性较大的邻苯二甲酸单丁酯葡萄糖醛酸结合物和邻苯二甲酸苄酯葡萄糖醛酸结合物. The metabolites of butylbenzyl phthalate in mice urine were identified by HPLC-MS^n . After the analysis of speculated metabolites and standard sample by HPLC-UV and the second full mass scanning （MS^2 ）, we find six metabolites： phthalic acid, benzoyl glycocoll, monobutyl phthalate, benzyl phthalate, glucuronyl-monobutyl phthalate, glucuronyl-benzyl phthalate. We deduced metabolic approach of butyl benzyl phthalate in vivo was that butyl benzyl phthalate hydrolyzed into the phthalic acid, monobutyl phthalate and benzyl phthalate. Partial phthalic acid became into benzoic acid by decarboxylizing. Then benzoic acid conjugated endogenous glycin and benzoyl glycocoll was produced. Monobutyl phthalate and benzyl phthalate conjugated endogenous β-D-glucuronic acid and produced the glucurony-monobutyl phthalate and glucuronyl-benzyl phthalate.
作者 李文兰 季宇彬 张大雷 范玉奇
机构地区 哈尔滨商业大学生命科学与环境科学研究发展中心 黑龙江省公安厅
出处 《环境科学》 EI CAS CSCD 北大核心 2007年第3期627-632,共6页 Environmental Science
基金 黑龙江省博士后基金项目
关键词 环境激素 邻苯二甲酸丁基苄酯 液相色谱/质谱法 代谢产物 environmental hormone butylbenzyl phthalate HPLC/MS metabolites
分类号 X830.2 [环境科学与工程—环境工程]
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参考文献21

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二级参考文献31

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环境科学
2007年 第3期

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