甲状腺功能减退对发育期鼠脑氧化应激状态的影响

Effect of hypothyroidism on oxidative stress status in developing rat brain

目的研究甲状腺功能减退(甲减)对发育期大鼠脑组织氧化应激状态的影响,进一步探讨甲减鼠脑发育异常的机制。方法以丙基硫氧嘧啶溶液灌胃,制成大鼠围生期甲减模型,收集出生后21 d的正常(对照组)及甲减幼鼠(甲减组)脑组织,测定脑组织匀浆液各项氧化应激指标的水平。结果甲减组幼鼠脑组织蛋白羰基及硫代巴比妥酸反应产物水平、总抗氧化力、还原型谷胱甘肽水平、超氧化物歧化酶及谷胱甘肽过氧化物酶的活性较对照组升高(P<0.05);过氧化氢酶活性两组间无统计学差异(P>0.05)。结论大鼠脑发育期甲减可引起脑组织氧化应激状态的改变,这可能参与了甲减性脑损伤的发生。

Objective To study the effect of hypothyroidism on oxidative stress status in developing rat brain and to further explore the mechanism of impaired brain development caused by hypothyroidism. Methods Perinatal hypothyroidism was induced by administering propylthiouracil （PTU） solution to the dams by garage. The oxidative stress indexes were measured in brain homogenate of normal and hypothyroid pups which were sacrificed on the 21st d after birth. Results As compared to the control, the following indexes were found to be increased in the hypothyroid group： protein carbonyl contents, thiobarbital acid reactive substances, reduced glutathione, total antioxidative capacity, activities of superoxide dismutase and glutathione peroxidase （P 〈 0.05）. However, there was no significant difference in the activity of catalase between the two groups （ P 〉 0.05）. Conclusion Hypothyroidism during rat brain development may cause oxidative stress, which may be related to the brain damage caused by hypothyroidism.