伯氏疏螺旋体与嗜吞噬细胞无浆体感染抑制Th1型细胞因子产生

Effect of coinfection with Borrelia burgdorferi and Anaplasma phagocytophilum on inhibition of production of Th1 cytokines in the murine host

目的应用动物模型探讨蜱传病原体伯氏疏螺旋体和嗜吞噬细胞无浆体混合感染对Th1型细胞因子产生的影响。方法建立三种伯氏疏螺旋体和嗜吞噬细胞无浆体混合感染小鼠模型和伯氏疏螺旋体单一感染小鼠模型。将小鼠分A组(同时感染组)、B组(伯氏疏螺旋体先于嗜吞噬细胞无浆体感染组)、C组(嗜吞噬细胞无浆体先于伯氏疏螺旋体感染组)、D组(伯氏疏螺旋体单感染组)和E组(无感染对照组)等共5组,每组16只,在不同时间点测定各组小鼠血清Th1型细胞因子浓度,并对数据进行统计学分析。结果与单一感染组相比,在螺旋体感染后18d和30d,三种混合感染组小鼠血清中Th1型细胞因子显著偏低。结论伯氏疏螺旋体和嗜吞噬细胞无浆体混合感染显著抑制Th1型细胞因子产生。

Objective To determine the effect of eoinfeetion of Borrelia burgdorferi and Anaplasma phagocytophilum on the production of Thl eytokines in the sera. Methods Eighty female C3H mice were randomly divided into 5 groups, including 3 eoinfeeted groups with Borrelia burgdorferi and Anaplasma phagocytophilum, 1 single Borrelia burgdorferi - infected group, and 1 negative control group. The mouse models co - infected with Borrelia burgdorferi and Anaplasma phagocytophilum and the single Borrelia burgdorferi - infected mouse model were established separately, Thl cytokines in the sera at two time points in different groups of mice were determined and compared statistically. Results Compared to the single - infected group, the level of Thl eytokine（IL- 2,IL- 12 and IFN - 7） in eoinfeeted groups significantly decreased 18th day and 30th day after Borrelia burgdorferi infection. Conclusion Co - infection of Borrelia burgdorferi and Anaplasma phagocytophilum can significantly inhibit the production of Thl eytokines in the murine host.