海南黎汉族高血压人群ACE基因多态性与危险因素研究

Study on the relationship between ACE gene polymorphismsand risk factors in hypertension patients of LI Nationality and Han Nationality in Hainan Province

目的研究海南黎汉族高血压人群ACE基因多态性及危险因素的关系。方法采用聚合酶链反应(PCR)方法,对海南黎族111例高血压患者、146例黎族正常人和海南汉族106例高血压患者、97例汉族正常人的ACE基因插入/缺失(I/D)多态性检测,观察DD、DI、II基因型频率及等位频率,并对所有普通PCR定为DD型的样本进行插入特异性PCR检测,以减少误分型率,并分析高血压患者经典危险因素。结果海南黎族高血压组与正常对照组比较,DD、DI、II基因频率及D、I等位基因频率均无显著性差异。海南汉族高血压组与正常对照组比较,DD、DI、II基因频率及D、I等位基因频率均差异有显著性(P<0.05)。ACE基因型分布与黎族、汉族高血压患者的年龄、性别、体重指数(BMI)、收缩压、舒张压、总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白(HDL-C)、载脂蛋白A(ApoA)、载脂蛋白B(ApoB)差异均无显著性(P<0.05)。结论ACE基因I/D多态性与黎族高血压无显著关联;ACE基因I/D多态性与汉族高血压有显著关联,是汉族高血压的主要致病基因;ACE基因型分布与黎族、汉族高血压经典危险因素无关,ACE缺失多态性是汉族高血压的独立危险因素。

Objective To investigate the relatioship between ACE gene polymorphisms and risk factors in hypertension patients of LI Nationalit and Han Nationality in Hainan Province. Methods The polymorphisms of ACE gene insertion/deletion （I/D） in 111 patients with hypertension and 146 healthy people of Li Nationality in Hainan and 106 patients with hypertension and 97 healthy people of Han Nationality in Hainan were determined. The frequencies of genotype and allele of DD, DI and II were also determined. Furthermore, insertion-specific PCR was used to test all the subjects who＇s DD genotype were determined by common PCR for confirmation Classical risk factors were also analyzed. Results There no significant differences both in genotype frequencies of DD, DI and Ⅱ, and in allele frequencies of D and Ⅰ between the hypertension patient Li Nationality and its healthy control were observed. But there were significant differences both in genotype frequencies of DD, DI and Ⅱ, and in allele frequencies of D and Ⅰ between the hypertension group of Han Nationality and its healthy control （ P 〈 0.05）. There no significant differences between the distribution of ACE genotype and age, body mass index （BMI）, systolic pressure, diastolic pressure, total serum cholesterol （TC）, triglyceride （TG）, LDL- C, HDL- C, ApoA or ApoB in hypertension patients of Li Nationality or Han Nationality （P 〈 0.05）. Conclusion There is no remarkable correlation between ACE gene I/D polymorphism and hypertension in Li Nationality, but I/D polymorphism of ACE gene is markedl correlated with hypertension patients of Han Nationality, and the I/D polymorphism of ACE gene is the major gene that causes hypertension in Han Nationality. The distribution of ACE genotype is no assoicated with classical risk factors of hypertension in Li and Han nationalities. The ACE deletion polymorphism is an independent risk factor for hypertension in Han Nationality.