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丝状真菌的DNA转化方法 被引量：9

DNA Transformation Method of Filamentous Fungi
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摘要 DNA遗传转化是人们进行菌种改良、有用基因克隆或基因功能研究的重要途径。丝状真菌的DNA转化方法有CaCl2/PEG介导的原生质体的转化、基因枪转化、电穿孔转化、限制酶介导(REMI)的转化、根癌农杆菌介导(ATMT)的转化等几种方法。 DNA genetic transformation is an important method to improve the strains, gene cloning and function gene assignment. Transformation methods of filamentous fungi have CaCl2/PEG Mediated Protoplasts Transformation, Biolistics, Electroporation, Restriction Enzyme Mediated Integration （REMI） and Agrobacterium Tumefaciens Mediated Transformation （ATMT）.
作者 苏彩云 靳发彬 张佳 丰枚枚 张春珍 王红怡 代明伟
机构地区 华北制药集团新药研究开发有限责任公司
出处 《河北化工》 2007年第7期29-31,共3页 Hebei Chemical Industry
关键词 丝状真菌 转化 DNA filamentous fungi transformation method DNA
分类号 Q785 [生物学—分子生物学]
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参考文献22

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二级参考文献66

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二级引证文献37

河北化工
2007年 第7期

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