开发缺氧调控载体用于肾性贫血的基因治疗

Exploitation of Hypoxia Regulatory Vectors for Gene Therapy of Renal Anemia

目的:开发具有缺氧调控活性的基因治疗载体,其表达可由缺氧选择性诱导,用于肾性贫血的逆转和治疗。方法:合成源于各种缺氧应答基因的缺氧应答元件(HRE)寡核苷酸序列,与CMV启动子连接组合,测定荧光素酶的相对活性以确定缺氧转录活性。结果:在各种缺氧调控载体中,由鼠PGK(mPGK)基因的HRE序列和CMV启动子组合成的缺氧应答启动子显示出14.6倍的高缺氧应答性,而且缺氧诱导的表达水平与完整的CMVI.E启动子在常氧环境下的转录水平相近。结论:3HRE/mPGK/CMV缺氧调控载体对于肾性贫血等一系列疾病实现目的基因的精确调控表达可能非常有用。

Objective： To exploit hypoxia regulatory vectors which express selectively under hypoxia and to utilize the vectors to reverse and treat renal anemia. Methods： Hypoxia response element（HRE） oligonucleotides derived from hypoxia response genes were synthesized and linked with CMV promoter to construct hypoxia response promoters. Then the hypoxic transcriptional activation of the constructs were measured by means of detecting relative luciferase activity. Results： Of all the vectors, the combination of 3HRE/mPGK and CMV promoter exhibited excellent hypoxia responsiveness （over 14-fold） to the similar level to the intact CMVI.E promoter under normoxia. Conclusion： To achieve gene therapy precisely regulated for a series of diseases such as renal anemia, the 3HRE/mPGK/CMV vector may be especially useful.