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SARS冠状病毒核壳蛋白对蛋白翻译的影响

Effect of SARS-CoV Nucleocapcid Protein on Protein Translation
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摘要 目的:研究SARS冠状病毒核壳蛋白(N蛋白)对蛋白翻译的影响。方法:构建N蛋白表达载体FLAG-pcDNA3-N,分别与FLAG-pcDNA3和表达荧光素酶的质粒共转染293T人胚肾细胞,通过检测荧光素酶的活性来判断N蛋白对细胞内蛋白翻译的影响;在体外翻译体系中检测N蛋白对体外翻译的影响。结果:构建了载体FLAG-pcDNA3-N,在293T人胚肾细胞内表达后,荧光素酶活性被抑制;在体外翻译体系中加入N蛋白,体外翻译被抑制。结论:SARS冠状病毒N蛋白抑制蛋白翻译。 Objective： To investigate the effect of SARS coronavirus nucleocapsid protein on translation. Methods： Construct the eukaryotic expression vector FLAG-pcDNA3-N. 293T cells were co-transfected with plasmids expressing FLAGpcDNA3-N or FLAG-pcDNA3 and luciferase. Investigate the effect of N protein in vivo through luciferase activity. The effect of N protein in vitro was examined in an RRL system. Results： The luciferase activity of lysate expressing FLAG- pcDNA3-N was lower than that expressing FLAG-pcDNA3. Addition of N protein in RRL system decreased luciferase activity. Conclusion： SARS coronavirus nucleocapsid protein could inhibit protein translation.
作者 周冰 曹诚 刘传暄
机构地区 军事医学科学院生物工程研究所
出处 《生物技术通讯》 CAS 2007年第2期193-195,共3页 Letters in Biotechnology
关键词 SARS冠状病毒 核壳蛋白 荧光素酶 蛋白翻译 SARS coronavirus nucleocapsid protein luciferase protein translation
分类号 Q75 [生物学—分子生物学]
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参考文献17

  • 1Zhong NS,Zheng BJ,Li YM,et al.Epidemiology and cause of severe acute respiratory syndrome (SARS) in Guangdong,People's Republic of China,in February,2003[J].Lancet,2003,362(9393):1353
  • 2Ksiazek TG,Erdman D,Goldsmith CS,et al.A novel coronavirus associated with severe acute respiratory syndrome[J].N Engl J Med,2003,348(20):1953
  • 3Drosten C,Gunther S,Preiser W,et al.Identification of a novel coronavirus in patients with severe acute respiratory syndrome[J].N Engl J Med,2003,348(20):1967
  • 4Rota PA,Oberste MS,Monroe SS,et al.Characterization of a novel coronavirus associated with severe acute respiratory syndrome[J].Science,2003,300(5624):1394
  • 5Marra MA,Jones SJ,Astell CR,et al.The genome sequence of the SARS-associated coronavirus[J].Science,2003,300(5624):1399
  • 6Rowland RR,Kervin R,Kuckleburg C,et al.The localization of porcine reproductive and respiratory syndrome virus nucleocapsid protein to the nucleolus of infected cells and identification of a potential nucleolar localization signal sequence[J].Virus Res,1999,64(1):1
  • 7Huang Q,Yu L,Petros AM,et al.Structure of the N-terminal RNA-binding domain of the SARS CoV nucleocapsid protein[J].Biochemistry,2004,43(20):6059
  • 8Surjit M,Liu B,Kumar P,et al.The nucleocapsid protein of the SARS coronavirus is capable of self-association through a C-terminal 209 amino acid interaction domain[J].Biochem Biophys Res Commun,2004,317(4):1030
  • 9Narayanan K,Maeda A,Maeda J,et al.Characterization of the coronavirus M protein and nucleocapsid interaction in infected cells[J].J Virol,2000,74(17):8127
  • 10Baric RS,Nelson GW,Fleming JO,et al.Interactions between coronavirus nucleocapsid protein and viral RNAs:implications for viral transcription[J].J Virol,1988,62(11):4280

二级参考文献13

  • 1SARS virus close to conclusive identification, new tests for rapid diagnosis ready soon SARS-multi-countryoutbreak-Update 12, Geneva:World Health Organization, 2003 (Accessed April 17 at http://www. who. int/csr/don/2003-03-27b/en/).
  • 2Situation in China and Hng Kng, status of diagnostic tests. SARS-multicountry outbreak-update 32. Geneva: World Health Organization, 2003(Accessed April 17at http://www.who.int/csr/don/2003-04-17/en/).
  • 3徐志凯,主编.实用单克隆抗体技术.西安:陕西科学技术出版社,1992.7-90.
  • 4J.萨姆布鲁克,E.F.弗里奇,T.曼尼阿蒂斯,著.分子克隆实验指南.第2版.北京:科学出版社,1992.880-898.
  • 5朱立平,陈学清,主编.免疫学实验方法.北京:人民军医出版社,2000.368-369.
  • 6Nelson GW, Stohlman SA, Tahara SM.High affinity interaction between nucleocapsid protein and leader/intergenic sequence of mouse hepatitis virus RNA. J Gen Virol,2000,81:181-188.
  • 7Chen H, Wurm t, Britton P, et al. Interaction of the coronavirus nucleoprotein with nucleolar antigens and the host cell. J Virol,2002,76:5233-5250.
  • 8Krokhin O,Li Y, Andonov A, et al. Mass spectrometric characterization of proteins from the SARS virus: a preliminary report. Proc Natl Acad Sci, 2004, 101:10036-10041.
  • 9Peiris JSM, Lai ST, Poon LLM, et al. Coronavirus as possible cause of severe acute respiratory syndrome. Lancet (Published online April 8,2003 http://image. the lancet. com/extras/03art3477 web. pdf).
  • 10Ksiazek TG, Erdman D, Goldsmith CS, et al. A novel coronavirus associated with severe acute respiratory syndrome. N Engl J Med, 2003,348:1953-1966.

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2007年 第2期

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