葡糖淀粉酶基因的分子生物学研究

The Research of Glucoamylase Gene in Molecular Biology

从酶活、底物、产物、应用及空间结构等方面论述了葡糖淀粉酶的一般生物学特性及应用,分析了其不同来源的基因同源度。着重从分子生物学角度阐述了葡糖淀粉酶基因在多种表达系统中的分泌表达情况,并对其基因在曲霉及酵母中的高效表达及分泌引导功能进行了初步阐述和探讨。

The general biology properties and applications of glucoamylase were discussed, and its enzymatic characteristics, such as substrates, products and structure were briefly introduced. The main discussions of this paper were the secrete expressions of the gene in different systerms, such as Aspergillus, yeast and E.coli, and the secrete inductive function of the gene in Aspergillus and yeast systerms.