GPRA基因rs324374位点基因多态性与儿童支气管哮喘的关系

Association between GPRA gene polymorphism and bronchial asthma in children

目的:探讨中国北方汉族儿童支气管哮喘易感性与哮喘相关G蛋白偶联受体(GPRA)基因多态性的关系。方法:采用PCR和限制性片段长度多态性(RFLP)方法检测中国北方117例支气管哮喘患儿和121例健康对照儿童GPRA基因rs324374位点基因多态性。结果:GPRA基因rs324374位点病例组和对照组中基因型分布均符合Hardy-Weinberg平衡定律;病例组rs324374位点的基因型频数分布:CC为22(18.80%),CT为66(56.40%),TT为29(24.80%),对照组该位点基因型频数分布分别为CC为30(24.79%),CT为61(50.42%),TT为30(24.79%),2组间基因型频数分布无显著性差异(P>0.05);C、T等位基因频数分布在2组间比较差异无显著性(P>0.05)。结论:GPRA基因rs324374位点等位基因突变与儿童支气管哮喘的发生没有明显的关联性。

Objective To investigate the association of susceptibility of bronchial asthma with GPRA gene polymorphism in North Chinese Han children. Methods The techniques of PCR and restriction fragment polymorphism （RFLP） were used to examine single nucleotide polymorphisms （SNPs） on rs324374 of GPRA gene in 117 children with bronchial asthma （ case group ） and 121 healthy individuals （ control group）. Results The genotype distributions on rs324374 of GPRA gene in case group and control group did not deviate from Hardy- Weinberg equilibrium. The frequency distribution of rs324374 in case group as follows： 22 were CC （18.80%）, 66 were CT （56.40%）, 29 were TT （24.80%）; the frequency distributions on rs324374 in control group as follows： 30 were CC （24.79%）, 61 was CT （50.42%）, 30 were TT （24. 79%） . And there was no significant difference of frequency distribution of three genotypes between case group and control group （P〉0. 05） and there was no significant difference of frequency distribution of allele C and T between two groups either （P〉 0. 05）. Conclusion The mutant allele of rs324374 of GPRA gene may not be associated with bronchial asthma in children.