电针对血清和大脑中肉毒碱的影响(英文)

EFFECTS OF ELECTROACUPUNCTURE ON CARNITINE IN SERUM AND BRAIN

目的:电针对于改善长期疲劳症状有一定的疗效,但是治疗机理尚不清楚,本实验旨在探讨其机理。方法:对电针组大鼠,针刺双侧肾俞,并进行捻转补泻1分钟。然后将针柄连接到疏密波电针仪上,频率为3 Hz ,通电30分钟,振幅正脉冲50 V,负脉冲25 V。电针治疗每天进行一次,连续五天。对照组(正常组)的大鼠,不采取针刺和电针刺激。治疗结束后的第二天杀死所有实验老鼠,检测它们的血液和大脑组织中的肉毒碱含量。结果:电针组和对照组(正常组)的大鼠血清内的肉毒碱含量分别为5.10±0.50μmol/L和2.17±0.46μmol/L;大脑组织内的肉毒碱含量分别为44.66±2.67μmol/L和24.05±3.65μmol/L。电针组血清和大脑组织中的肉毒碱含量明显高于对照组(P<0.001)。结论:本研究显示电针可以明显提高血液和大脑中肉毒碱的含量,表明电针对能量代谢和改善疲劳具有影响。

Objective Electroacupuncture （EA） has been shown to have therapeutic effect on chronic fatigue syndrome. But its action has not been made clear. Methods After the rats of EA group were fixed in an animal cage, bilateral Shènshū （肾俞 BL23） were punctured and stimulated with uniform reinforcing and reducing method by twirling the acupuncture needle for one minute. And then the needle handles were connected to an electric stimulator for stimulating the acupoint with dense-sparse waves, frequency of 3 Hz for 30 minutes, and amplitude： positive： 50 V, negative： 25 V. Electrocupuncture was given once a day, continuously for 5 days. The rats of normal control group were not punctured and stimulated. The rats of all groups were killed for collecting blood and brain tissue on the next day after the final treatment. Carnitine in serum and brain tissue was determined. Results Oarnitine contents in serum of EA group and of normal control group were 5.10±0.50 μmol/L and 2.17±0.46 μmol/L, respectively. Oarnitine contents in brain tissue of elctroacupuncture group and of normal control group were 44.66 ± 2.67 μmol/L and 24.05 ± 3.65 μmol/L, respectively. Oarnitine levels in serum and brain of EA group were significantly higher than those of normal control group. （P〈0.001） Conclusion This report presented that carnitine in serum and brain is increased by EA, suggesting that EA may affect energy metabolism and may have effects on fatigue.