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外源性HPV16 E7癌基因表达对宫颈癌HeLa细胞Cyclin E表达的影响 被引量：2

INFLUENCE ON EXPRESSION OF Cyclin E IN HeLa CELLS BY EXPRESSION OF THE EXOGENOUS HPV16 E7 ONCOGENE
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摘要 目的研究重组腺病毒介导人乳头状瘤病毒(HPV)16型E7病毒癌基因感染对人宫颈癌HeLa细胞周期蛋白E(Cyclin E)表达的影响,探讨HPV16 E7癌基因以及Cyclin E在宫颈癌发生发展中的作用。方法用含有HPV16 E7病毒癌基因的重组腺病毒载体感染体外培养的人宫颈癌HeLa细胞。免疫荧光染色后激光扫描共聚焦显微镜(LSCM)检测感染前后Cyclin E表达的差异。结果HPV16 E7感染后Cyclin E表达较感染前明显增加。结论HPV16 E7基因的表达促进Cyclin E的表达,对宫颈肿瘤细胞有增殖促进的作用。 Objective To investigate the expression of Cyclin E in HeLa cells after the transfection of HPV16 E7 oncogene by recombinant adenovirus vector, and assess the effect of HPV16 E7 oncogene and Cyclin E in the development of human cervical carcinoma. Methods Expression of Cyclin E was detected by laser scanning confocal microscope （LSCM） before and after the transfection. Results Expression of Cyclin E protein in the HeLa cells increased significantly after the transfection of HPV16 E7 genes. Conclusion Expression of HPV16 E7 oncogene influences the expression of Cyclin E and enhances the proli feration of the cells of cervical carcinoma.
作者 田甜 戴淑真 孔守芳 黄丽丽 罗兵
机构地区 青岛大学医学院附属医院妇科 青岛大学医学院微生物学教研室
出处 《青岛大学医学院学报》 CAS 2007年第3期210-211,214,共3页 Acta Academiae Medicinae Qingdao Universitatis
关键词 乳头状瘤病毒 人 基因 E7 感染 宫颈肿瘤 细胞周期蛋白E papillomavirus, human genes, E7 infection cervix neoplasms Cyclin E
分类号 R737.33 [医药卫生—肿瘤]
  • 相关文献

参考文献11

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共引文献23

同被引文献18

  • 1戴淑真,李旭日,王宁,罗兵.宫颈癌组织中P16和HPV16/18E7的表达及意义[J].齐鲁医学杂志,2004,19(4):283-286. 被引量：6
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二级引证文献3

青岛大学医学院学报
2007年 第3期

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