血浆MMP2与TIMP1含量及比值对膀胱癌转移意义

PLASMA MMP2,TIMP1 AND MMP2/TIMP1:ITS DIAGNOSTIC VALUE FOR BLADDER CARCINOMA

目的探讨血浆基质金属蛋白酶-2（MMP2）、金属蛋白酶组织抑制因子-1（TIMP1）的含量及MMP2/TIMP1比值对膀胱癌的诊断价值及其与膀胱癌侵袭转移的关系。方法采用ELISA法检测76例膀胱移行细胞癌病人和30例健康成人血浆中MMP2、TIMP1的含量。结果膀胱癌有淋巴结转移组和无转移组血浆中MMP2的含量及MMP2/TIMP1比值均明显高于对照组（H=71.62、16.15,t=2.84-5.14,P〈0.01）,TIMP1含量明显低于对照组（H=42.61,t=3.67,P〈0.01）;有淋巴结转移组MMP2的含量高于无淋巴结转移组（t=2.03,P〈0.05）,MMP2/TIMP1比值差异无显著性（P〉0.05）。结论血浆MMP2、TIMP1的含量及其比值对膀胱癌的诊断具有重要价值,MMP2含量及MMP2/TIMP1比值可作为膀胱癌侵袭转移的辅助判断指标。

Objective To assess the diagnostic value of plasma matrix metalloproteinase 2 （MMP2）, tissue inhibitor of metalloproteinase 1 （TIMP1） and their ratio in transitional cell carcinoma of bladder and their relation with the invasion and metas tasis of the cancer. Methods The plasma levels of MMP2 and TIMP1 were detected using sandwich enzyme-linked immunosorbent assay （ELISA） in 76 patients and 30 healthy controls. Results MMP2 and the ratio of MMP2/TIMP1 were significantly higher in patients than those in the healthy controls （H=71.62,16.15.t=2. 84-5.14.P〈0. 01）, while TIMP1 was significantly lower in the patients （H=42.61, t=3.67, P〈0. 01）, The levels of MMP2 were elevated in the metastatic compared with that of the zero-metastatic （t=2. 03, P〈0.05）. Conclusion The plasma levels of MMP2, TIMP1 and the ratio of MMP2/TIMP1 are of great importance in the diagnosis of transitional cell carcinoma of bladder. Furthermore, the MMP2 and the ratio of MMP2/ TIMP1 may serve also as a supplementary parameter for its metastasis.