Fas和Ki-67在非小细胞肺癌组织中的表达

EXPRESSION OF Fas AND Ki-67 IN NON-SMALL CELL LUNG CANCER

目的 探讨Fas和Ki-67在非小细胞肺癌（NSCLC）组织中的表达及临床意义。方法 采用免疫组化EnVison法检测Fas和Ki-67在60例NSCLC及18例癌旁正常肺组织中的表达。结果 在NSCLC组织中，Fas表达率显著低于癌旁正常肺组织（χ^2=6．94、14．30，P〈0．05），并与NSCLC的病理分级、临床分期及淋巴结转移有关（χ^2=7．17～10．90，P〈0．05）；Ki-67表达率显著高于癌旁正常肺组织（χ^2=18．80、14．20，P〈0．01），并与NSCLC的病理分级、临床分期及淋巴结转移有关（=8．47～13．10，P〈0．05）。分化程度低、已发生转移的肺癌Fas表达水平显著降低，而Ki-67表达水平显著升高。在NSCLC组织中，Fas和Ki-67表达水平呈负相关（χ^2=3．93，P〈0．05）。结论 Fas和Ki-67表达异常与肺癌发生、发展及转移关系密切，对判断肺癌恶性程度及预后有重要参考价值。

Objective To observ the expression of Fas and Ki-67 in non-small cell lung cancer （NSCLC） and discuss the related clinical significance. Methods EnVison immunohistochemical method was applied to investigate the expression of Fas and Ki-67 in the tissue of NSCLC （n= 60） and the normal neighboring lung tissue （n=18）. Results The level of Fas expression in NSCLC was significantly/ower than that in adjacent normal tissues （χ^2=6.94,14.30; P〈0. 05）, and was correlated with pathological grading, clinical staging and lymphatic metastasis （χ^2=7.17- 10.90,P〈0.05）. The level of Ki-67 expression in NSCLC was significantly higher than that in adjacent normal tissues （χ^2= 18. 80,14.20 ; P〈0.01 ）, and was also correlated to pathology （χ^2=8.47- 13.10,P〈0.05）. NSCLC in the advanced stage with poor differentiation and lymph node metastasis possessed lower Fas expression but higher Ki-67 expression （χ^2=3.93,P〈0.05）. Conclusion Abnormal expression of Fas and Ki-67 is related to the occurrence, development, whether or not metastasis of NSCLC, and is of importance in the evaluation of malignancy and prognosis of the disease.