逆转录聚合酶链反应检测蚊体内黄病毒的研究

Study on Detection of Flavivirus RNA in Mosquito by Reverse-transcriptase Polymerase Chain Reaction

本文报告了应用自行设计的黄病毒通用引物和逆转录聚合酶键反应成功地扩增了实验室脑内接种感染白纹伊蚊体内登革病毒RNA和海南省非流行区野外捕获的白纹伊蚊和骚扰阿蚊体内黄病毒RNA。该法灵敏度高，特异性强，获得结果迅速。能检出5只蚊虫中含有1只阳性蚊的混合标本中的登革病毒RNA。整个过程只要数小时就可完成。这对蚊体内黄病毒的快速检测及流行病学调查、防治监测等研究，具有重要意义。

This paper reports that dengue viral RNA from intrathoracially-infected mosquito,Aedes albopictus,and flavivirus RNAs from field-caught mosquitoes(Aedes albopictus and Armigeres subalbatus ) in non-epidemical district of Hainai,were succesfully amplified with flavivirus-specific universal primers by reverse transcriptase-polymerase chain reaction. The method was more sensitive and specific than the traditional ones. Dengue 2 viral RNA could be detected from one infected mosquito in a pool of five. All the procedure could be completed within a few hours.