武夷湍蛙胃肠道内分泌细胞的免疫组织化学定位(英文)

IMMUNOHISTOCHEMICAL LOCALIZATION OF ENDOCRINE CELLS IN THE GASTROINTESTINAL TRACT OF MT. WUYI CASCADE-FROG(AMOLOPS WUYIENSIS)

用免疫组织化学S-P法,应用七种特异性胃肠激素抗血清对武夷湍蛙胃肠道7个部位内分泌细胞的局部分布、分布密度及形态学特征进行了研究。结果显示:在武夷湍蛙胃肠道中鉴别出四种内分泌细胞,即:5-羟色胺、生长抑素、胃泌素和高血糖素免疫反应细胞。P-物质、胰岛素和胰多肽免疫反应细胞未见。5-羟色胺免疫反应细胞是胃肠道中最主要的内分泌细胞类型,其在胃肠道各段均有分布,但在各段分布密度不同。生长抑制素免疫反应细胞分布于贲门至十二指肠的胃肠道各段。胃泌素免疫反应细胞局限分布于幽门和十二指肠部位。高血糖素免疫反应细胞仅在胃幽门部位有分布。在武夷湍蛙胃肠道内具有最多种类型内分泌细胞分布的部位是幽门,同时胃肠道内各种内分泌细胞在此部位也显示出最高的分布密度。武夷湍蛙胃肠道内分泌细胞形态多样:圆形、椭圆形、纺锤形、梭形、锥形和不规则形。胃部多数内分泌细胞分布于胃腺中,肠道中多数细胞则分布于上皮细胞间,少数分布于固有膜。本研究显示武夷湍蛙与其他两栖动物胃肠道内分泌细胞在分布模式上存在一定共同特征并具其独特性,以上结果提示其与武夷湍蛙消化生理学相关,和其胃肠道的调节特点相关。

Using 7 types of antisera, the regional distribution and distributional density as well as morphological features of endocrine cells in 7 parts of the gastrointestinal track （GIT） of Mt. Wuyi cascade-frog （ Amolops wuyiensis ） were investigated by streptavidin- peroxidase （S-P） immunohistochemical method. The result showed that four types of gastrointestinal （GI） endocrine cells were identified in the GIT of A. wuyiensis, e.g. 5-hydroxytryptamine （5-HT）, somatostatin（SS）, gastrin（GAS）, glucagon （GLU） and immunoreactive（IR） cells. Substance P （SP）-, Pancreatic Polypeptide （PP）- and Insulin （INS）-IR cells were not detected. 5-HT-IR cells distributed throughout the whole GIT at various densities and they were the most predominant cell types in the GIT. SS-IR cells were detected from cardia to duodenum. GAS-IR cells showed a restricted distribution and were only demonstrated in the pylorus and duodenum. GLU-IR cells were only confined to pylorus. In the GIT of A. wuyiensis, the region with the highest degree of cell type heterogeneity was pylorus and all types of endocrine cell along the GIT showed peak density in pylorus as well. The endocrine cells were round or oval, spindle, shuttle, pyramid or irregularity in shape. And they were mostly found in the gastric glands of the stomach regions and in the epithelium of intestine, and occasionally occurred in lamina propria. The present study showed that some common features on the distributional pattern of the different types of GI endocrine cells were found between A. wuyiensis, and other amphibians, and on the other hand, some species-dependent unique distributional patterns of endocrine cells were also detected in GIT of A. wuyiensis. The results suggested that these characteristics might be related to the GIT physiology and the regulatory characteristics of the GIT of the A. wuyiensis.