摘要
选择5%聚乙烯醇为膜材料,与10u/ml胰岛素抗体混匀,在压电石英晶体上制备活性抗体膜。放免法检测结果表明,抗体膜具有良好的机械强度、透性和响应性能。抗体膜与不同浓度的胰岛素抗原结合,显示抗体膜上胰岛素结合量与胰岛素浓度呈线性相关。
Active antibody membrane on piezoelectric quartz crystal was prepared with the mixture of 5% polyvinyl alcohol(PVA) and 10 u/ml insulin antibody. The results measured by radioimmunoassay and acoustic immunosensor show that there are better functions in the strength, responsibility, and penetration of the membranes . The binding amount of insulin to the certain concentration of antibody immobilized in the membrane is better correlative to the concentration of insulin in samples.
出处
《中国药科大学学报》
CAS
CSCD
北大核心
1997年第1期52-52,共1页
Journal of China Pharmaceutical University
基金
江苏省科学技术委员会资助