痰培养及血清学联合检测肺炎衣原体性急性呼吸道感染

Assessment of sputum cultre and serology for detection of chiamydia pneumoniae in patients with sympeomatic acute respiratory tract infection

目的为了解成人急性呼吸道感染患者肺炎衣原体感染情况，为临床治疗提供参考。方法对９６例患者及４８例健康献血员同时行显微免疫荧光检测肺炎衣原体抗体，前者３７例同时行痰ＨＥＰ－２细胞培养分离肺炎衣原体。结果２６例患者（２９％）血清学ＩｇＧ≥１：５１２或ＩｇＭ≥１：３２提示急性肺炎衣原体性呼吸道感染，其中以急性支气管炎及支气管哮喘急性发作多见（分别为３５．８％及３０．０％）。对照组无一例出现急性感染血清学滴度。痰培养８例阳性（２１．３％，８／３７）经兔抗鼠免疫荧光染色确诊为肺炎衣原体。结论成人肺炎衣原体性急性呼吸道感染并非少见，应重视肺炎衣原体检测并给予特殊处理。

Objective To evaluate sputum culture and serology for detection of chlamydia pneu moniae(C.pneumoniae)in adult patients with acute respiratory tract infection and normal subjects.Methods Microimmunoflorescence(micro IF)test for IgG and IgM antibodies to C.pneumoni ae spe cific antigen was performed in every subject.Thirtyseven patients also had sputum HEP 2cell culture tested for C.pneumoniae.ResultsTwenty six patients(29.7%)with micro IF IgG titres equal to or greater than1:512or/and IgM titres equal to or greater than1:32 indicated recent infection.Eig ht pa tients(21.3%)with positive sputum culture of C.pneumoniae were identified.No similar titer was shown in control normal subjects.ConclusionsAcute infection of C.pneumoniae were common in symptomatic patients with acute respiratory tract infection and attention should be drawn to this special illness.