羟基磷灰石复合骨形态发生蛋白2人工骨的理论及其应用特征

Bone morphogenetic protein 2 comprising hydroxyapatite artificial bone

目的:系统回顾当前国内外关于骨形态发生蛋白2复合羟基磷灰石人工骨的研究进展。资料来源:应用计算机检索Pubmed 1965-01/2007-03关于骨形态发生蛋白2符合羟基磷灰石人工骨的文章。检索词“BMP-2,hydroxyapatite”并限定文章的语言种类为English。同时利用计算机检索万方数据库2002-09/2005-07的相关文章,限定文章语言种类为中文,检索词“骨形态发生蛋白2,羟基磷灰石”。资料选择:纳入标准:①骨形态发生蛋白2复合羟基磷灰石人工骨的动物实验及临床运用。②骨形态发生蛋白2的分子生物学,成骨机制。③羟基磷灰石成骨作用。④能获取文章的全文。排除标准:①较陈旧文献。②重复性研究。资料提炼:共收集到50篇与骨形态发生蛋白2复合羟基磷灰石人工骨有关的文章,45篇符合纳入标准。其中研究内容相似的,以近7年发表在较权威杂志者优先。排除的15篇为较陈旧的文献及重复研究;对符合标准的30篇文献进行分析。资料综合:由于骨形态发生蛋白2弥散能力较强,单独植入体内会很快弥散被吸收,需要一种合适的载体吸附骨形态发生蛋白2,而羟基磷灰石具有与松质骨相类似的多孔支架结构,无抗原性,为理想的骨形态发生蛋白载体系统。虽然此种材料的应用尚处于实验阶段,但应用前景广阔,其必将成为具有主导性的人工骨材料。结论:生物组织工程学的发展,为骨缺损的修复提供了契机,也提出了挑战,即要求有一种生物相容性更好、又满足生物力学需要的载体材料。在这一方面骨形态发生蛋白2复合羟基磷灰石人工骨有着广阔的研究、开发前景。

OBJECTIVE： To systematically review the research development in bone morphogenetic protein 2 （BMP-2） comprising hydroxyapatite artificial bone at home and abroad in recent years. DATA SOURCES： A computer-based online search of Pubmed database was undertaken to identify the English articles about BMP-2 comprising hydroxyapatite artificial bone published from January 1965 to March 2007 by using the key words of ＂BMP-2, hydroxyapatite＂. Meanwhile, Wanfang database was searched for the Chinese relevant articles published between September 2002 aro Jury 2005 with the same key words in Chinese STUDY SELECTION： Inclusive criteria： ①the animal experiment and clinical application of the BMP-2 comprising hydroxyapatite artificial bone. ②the biochemistry of BMP-2 and its bone-producing mechanism. ③the osteogenesis of hydroxyapatite④the articles with accessible full-texts. Exclusive criteria： ①obsolescent articles②repetitive articles. DATA EXTRACTION： Totally 50 relevant articles were collected and 45 of them were selected in accordance with the inclusive criteria. As for the researches with similar contents, those published in authoritative journals in the past 7 years were firstly selected. And 15 repetitive and old articles were excluded, 30 literatures in accordance with the criteria were analyzed. DATA SYNTHESIS： BMP-2 is absorbed quickly when used alone because of its powerful dispersion, so it needs an appropriate carrier to absorb BMP-2. Hydroxyapatites have the porous construction like the spongy bone, and have no antigenity, so it is a perfect carrier system of BMP. The artificial bone will be the mastered materials, although it is at the experimental phase. CONCLUSION： With the devetopment of the biochemistry and tissue engineering, the repair of the bone defect faces a new era, also arises a challenge that the materials need a carrier of good biocompatibility and mechanical properties. At this point, BMP-2 comprising hydroxyapatite artificial bones have the promising application.