兔肌腱细胞和成纤维细胞与人工材料体外联合培养的形态学观察

MORPHOLOGICAL OBSERVATION OF COMBINED CULTURE OF TENDON CELL OR FIBROBLAST OF RABBIT WITH ARTIFICIAL MATERIALS IN VITRO/

在兔肌腱细胞、成纤维细胞分离培养的基础上，将冻存复苏的传代细胞与碳纤维、涤纶及几丁质三种材料体外联合培养，观察细胞与材料的相容性以及细胞在材料上的生长情况，比较这两种细胞在三种材料上的生长差异。结果发现，在体外培养条件下，碳纤维与两种细胞的相容性均好，有良好的吸附性；涤纶材料上两种细胞生长均少，可能与其表面结构有关；几丁质明显抑制这两种细胞生长。同时发现，无论肌腱细胞，还是成纤维细胞，在碳纤维上座落的数量都明显优于涤纶、几丁质。三种材料交错在一起时，胶原纤维方向是以碳纤维为轴，形成网状，交织缠绕在材料之间。随时间延长，细胞在单根碳纤维或多根碳纤维上排列均匀，包绕碳纤维，且相互衔接。研究结果，为进一步研究有生命的人工肌腱、韧带提供了依据。

In order to investigate the compatibility and growth between the tendon cell or fibroblast of rabbit and artificial materials, the combined culture of the two cells with the carbon fiber, terylene and chitin was observed respectively. Results showed as following: in vitro, the compatibility of carbon fiber with these two cells was well, cell adhesion ability was good as well. Few cells grew on terylene. Chitin inhibited the growth of either cells. No matter the tendon cell or the fibroblast, the amount of cells adhering on the carbon fiber was far more than that on terylene or chitin. When the three materials were interlaced together, the collagen fibers produced by the cells were arranged in direction parallel to the carbon fibers. As the time elapsed, the cells on the carbon fiber distributed evenly and enveloped the material in network like fashion, this suggested that carbon fiber was a good material for producing living artificial tendon and ligament.