摘要
目的:确定银翘伤风胶囊质量标准。方法:通过薄层鉴别方法(TLC)对处方中金银花、牛黄、芦根、甘草进行定性鉴别。运用高效液相色谱法(HPLC)对连翘中的连翘苷进行含量测定。结果:薄层色谱斑点清晰,阴性对照无干扰。含量测定连翘苷量在0.199-0.991μg之间有良好的线性关系,相关系数r=0.999 9。回收率为99.7%,RSD=0.48%(n=5)。结论:所建立的方法可靠,能准确地进行定性、定量检测,可用于该制剂的质量控制。
Objective: To establish the quality standard for Yinqiaoshangfeng capsules. Methods: Flos Lonicerae, Calculus Bovis, Rhizoma Phragmitis and Radix Glycyrrhizae were identified by TLC. Forsythin was determined by an HPLC method. Results: TLC spots clear and specific. A good linear relationship between peak area and concentration of forsythin was found at the range of 0.199 - 0.991 μg with a correlation coefficient of r = 0.999 9. The average recovery of forsythin was 99.7 % and RSD was 0.48 % ( n = 5 ). Conclusion: The method is reliable, accurate and specific. It can be used for the quality control of Yinqiaoshangfeng Capsules.
出处
《中国实验方剂学杂志》
CAS
2007年第2期6-9,共4页
Chinese Journal of Experimental Traditional Medical Formulae