摘要
目的:应用高效液相色谱(HPLC)法测定荣骨颗粒中淫羊藿苷的含量,控制该制剂的质量。方法:采用Zorbax-SB-C18柱(5μm,4.6×150 mm)分离淫羊藿苷,以甲醇-水(60∶40)为流动相,检测波长为270 nm。结果:淫羊藿苷线性范围为0.1-1.0μg,r=0.999 9,平均回收率为101.7%,RSD=1.86%(n=5)。结论:该方法简便、可靠、准确,可用于该制剂的质量控制。
Objective: To determine the content of Icariin in Ronggu Granule by HPLC for quality control. Methods :Zorbax-SB-C18(5 μm,4.6 × 150 mm) column was used with methanol: water (60:40)as mobile phase, and the determination wavelength was set at 270 nm. Results: The linear range was 0.1 - 1.0 μg for Icariin( r = 0.999 9), the average recovery was 101.7% and RSD was 1.86% (n = 5). Conclusion:This method is simple, reliable and accurate for the determination of icariin, it can be used for quality control of this preparation.
出处
《中国实验方剂学杂志》
CAS
2007年第2期10-11,共2页
Chinese Journal of Experimental Traditional Medical Formulae