熟地、苍术及两者不同配伍比例保护大鼠残余肾和抑制转化生长因子的实验研究

The Experimental Study of Shu Di,Cang Zhu and Different Compatibility of Them to Protect Rat's Remnant Kidney and Inhibit Transforming Growth Factor

目的:观察熟地、苍术及二者不同配伍比例保护大鼠残余肾和抑制转化生长因子β1的作用,并寻找熟地、苍术的最佳配伍比例。方法:采用大鼠5/6肾切除动物模型,分为单味熟地组、熟地伍苍术(1∶1)组、熟地伍苍术(2∶1)组、熟地伍苍术(1∶2)组、单味苍术组、模型空白对照组、正常对照组,观察各组大鼠治疗前后一般状况、血红蛋白(Hb)、肾功能(血清肌酐,Scr)、转化生长因子β1(TGFβ1)的表达。结果:单味熟地组、熟地伍苍术(1∶1)组、熟地伍苍术(2∶1)组与模型组相比有较大差异(P<0.05)。组间比较:熟地伍苍术(1∶1)组与单味熟地、熟地伍苍术(1∶2)、熟地伍苍术(2∶1)、单味苍术组间有差异(P<0.05)。结论:单味熟地、熟地伍苍术(1∶1)、熟地伍苍术(2∶1)均有抗肾纤维化的作用,尤以熟地伍苍术(1∶1)疗效最佳。

Purpose ： To observe the protective effect of Shu Di, Cang Zhu and their compatibilities on rat model of remained kidney;and to figure out the best compatibility of the two herbs for their effect and for inhibiting transforming growth factor β1 of the animal. Methods：Rat model was established by removing 5/6 kidney. The model rats were divided into the following groups：Shu Di group,Shu Di ＋ Cang Zhu（1： 1） group,Shu Di ＋ Cang Zhu（2： 1）group,Shu Di ＋ Cang Zhu（1：2） group, Cang Zhu group, and model control group. Additionally, a control group of normal rats was also used. Before and after the experiment, the general condition, hemoglobin（ HGb）, renal function（ Scr）, expression of transforming growth factor β1 of the animals were observed and determined. Results ： Shu Di group, Shu Di ＋ Cang Zhu（ 1 ： 1 ） group and ShuDi ＋ CangZhu（2：1 ）group were significantly different from the model control（ P 〈 0.05）. There were also significant differences between Shu Di ＋ Cang Zhu（1：1 ） group and the following groups： Shu Di group, Shu Di ＋ Cang Zhu （2：1 ） group, Shu Di ＋ Cang Zhu（ 1 ： 2） group and Cang Zhu group（ P 〈 0.05 ）. Conclusions ： Shu Di group, Shu Di ＋ Cang Zhu （ 1 ： 1 ）, Shu Di ＋ Cang Zhu （2 ： 1 ） appears to have the function of antifibrosis to kidney, especially the compatibility of Shu Di ＋ Cang Zhu（1：1）.