3种方药对脑出血大鼠因子Ⅷ相关蛋白和胶质纤维酸性蛋白的影响

Effects of Three TCM Preparations on vWF and GFAP of Rats with Cerebral Hemorrhage

用成年Wistar大鼠脑内注射胶原酶法制作脑出血模型 ,观察中药醒脑健神胶囊、清开灵注射液和中风脑得平冲剂对脑出血大鼠脑内皮细胞的因子Ⅷ相关蛋白(vWF)及胶质细胞内的胶质纤维酸性蛋白 (GFAP)的影响。结果给药组与对照组比较vWF阳性新生血管增多 ,GFAP阳性细胞胞体明显增大 ,纤维显著增粗。提示 3种方药皆可激活内皮细胞和胶质细胞 ,促进坏死组织吸收。

Adult rats with cerebral hemorrhage induced by injecting collagenase intracerebrally were used in the study. The effects of Xingnao Jianshen Capsules, Qingkailing Injection and Zhongfeng Naodeping Granules on vWF and GFAP of the rats with cerebral hemorrhage were studied by the immunohistochemical method. The results showed that compared with the control group, the number of vWF positive neogenetic vessels was increased, the bodies of GFAP positive cells were enlarged and the fibers of the same cells were thickened,which implies that these three TCM preparations can activate endothelial cells and gliocytes, and promote the absorption of necrotic tissue, proliferation of vessels and repair of cerebral tissue.