神经节苷脂对体外培养的嗜铬细胞的影响

EFFECT OF THE GANGLIOSIDES ON PHEOCHROMOCYTES IN VITRO CULTURE

将新生ＳＤ大鼠肾上腺髓质中的嗜铬细胞在含有神经节苷脂的培养液中培养，应用相差显微镜、荧光组织化学及免疫细胞化学染色等方法，观察嗜铬细胞的成活及突起生长情况。实验结果表明，神经节苷脂可明显提高嗜铬细胞的成活率。

Pheochromocytes from the neonatal rat adrenal medulla were cultured in the presence of gangliosides.The survival of pheochromocyte and its process were studied with phase contrast microscope,fluorescent histochemical and immunocytochemical staining techniques.The results showed that gangliosides could markedly increase the survival rate of pheochromocytes in culture.