人骨折骨痂中转化生长因子β_1的免疫组化研究

Immunohistochemical Localization of TGF-β 1 in Human Fracture Callus

为了解转化生长因子β１（ＴＧＦ－β１）在人骨折骨痂中的细胞定位，表达强度及其与骨折修复的关系，采用不同修复阶段的人骨折骨痂组织切片，以ＴＧＦ－β１抗体进行免疫组化研究。结果发现：成骨细胞、新骨骨基质、修复中的关节软骨细胞中ＴＧＦ－β１染色强阳性，肥大的软骨细胞染色阴性。改建期骨破骨细胞染色阳性。染色强度在１４～２６２天中逐渐减弱。骨不连组织ＴＧＦ－β１染色阴性。ＴＧＦ－β１可能由人成骨细胞合成并分泌入基质，并自骨折后的较早期直至改建期持续发挥重要作用。

For the investigation of the cellular localization of transforming growth factor β 1 ( TGF- β 1) in human fracture callus and the relationship between the localization and healing of the fracture, human fracture callus at different stages and TGF- β 1 antibody were adopted.Results showed that the osteoblasts, bone matrix and articular cartilage in repairing process were strongly stained with this antibody.The osteoclasts in remodeling bone were also stained positive. The staining for differentiated (hypertophic) chondrocytes and in tissue of the nonunion were negative. The results indicate that TGF-β 1 can be produced by osteoblasts and secreted into the bone matrix, and is a regulator for human fracture repair from early stage to the remodeling of woven bone.