不同种源乌拉尔甘草形态和ISSR遗传多样性研究

Genetic Diversity of Glycyrrhiza uralensis Fisch. Detected by Morphological and ISSR Molecular Markers

利用形态学指标对34份乌拉尔甘草种质资源进行遗传多样性分析,结果表明,34份甘草在7个性状上差异均达到了极显著水平,主要是由以内蒙古和新疆种源为主的两大组群之间形态特性差异明显。在分子标记中,从100条核甘酸序列中筛选出18条ISSR多态性引物,对46份乌拉尔甘草种质资源进行了遗传多样性分析,研究结果表明：①我国乌拉尔甘草种质资源遗传变异十分丰富,18条引物共扩增出210条多态性带,分布在150~3000bp之间,平均每个引物扩增出11.67条;②新疆地区乌拉尔甘草遗传变异最为丰富,其次是西北地区,东北地区遗传变异最低;③基于ISSR分子标记划分的组群与地域性没有明显关系。

Morphological analysis were carried out on 34 accessions of Glycyrrhiza uralensis Fisch. from different regions. Results showed that the difference of morphological characters among 34 accessions reached significant level. Thirty-four accessions were mainly divided into 2 groups： Xinjiang and Inner Mongolia, and the differences of morphological characters between 2 groups were significant. Genetic diversity of 46 accessions of Glycyrrhiza uralensis Fisch. was detected by 18 ISSR primers which screened from 100 primers, and the results were as followed： （1） Glycyrrhiza uralensis Fisch. accessions possessed high genetic diversity. A total of 210 polymorphic bands were obtained, in average 11.67 polymorphic bands produced by each primer. （2） Genetic diversity of Xinjiang region was most abundant, the second was North-western region, and North-eastern region was the least. （3） Groups based on ISSR markers were not in evidence with geographical regions.