肝外胆管癌细胞系的建立

Establishment of a cell line of extrahepatic bile duct carcinoma

为研究胆管癌生物学特性，我们建立了国内首株人肝外胆管癌细胞系，命名为ＱＢＣ＿９３９，组织学上与源癌组织相似。观察结果：ＱＢＣ＿９３９细胞具有较多的微绒毛、微管、微丝及细胞连接，细胞质和细胞膜存在角蛋白和桥粒蛋白等上皮来源特征，且生长速度快，群体活力高，独立生存能力强，并具有较强的致瘤性；该细胞系呈现明显的染色体数目和结构异常，频繁发生断裂、移位、缺失和插入等，多倍体癌细胞比例甚高。结果表明：该细胞系的建立对胆管癌细胞生物学特性研究提供了有价值的资料，并且为进一步的研究提供了稳定、可靠的细胞来源。

To investigate the cellular biological charateristics of bile duct carcinoma, the first cell line .of extrahepatic bile duct carcinoma ,named as QBC939 , was established in .China. The cell line was histologically sim- ilar to the originai tumor. Electron microscopic studies indicated the presence of characteristic junctions be- tween cells , microvilli on the cell surface , microfilaments and microtube in cytoplasm. Immunohistochemistry of cultured cells and reconstituted tumor in nude mouse showed that the cell line was strongly positive for karatin and desmosome , supporting the epilhelial origin of .the cell line. The study of growth kinetics demon- strated that the QBC939 cell line grew fast with inde- pendent and higher viability. The cytogenetic study re- vealed that the abnormality in number and structure , such as fracture , transplantation , loss and insert of chro- mosomes , presented in the cell line. Soft sepharose cul- ture and nude mouse xenografts indicated that this cell line was strungly tumorigenic.