一氧化氮在大白鼠肝硬变门静脉高压症中的作用

Role of nitric oxide in rets with hepatocirrhosisand portal hypertension

为探讨一氧化氮在肝硬变形成过程中的作用，我们通过观察一氧化氮合成酶在正常鼠和肝硬变、门静脉高压症大鼠肝脏的分布，同时测定了门静脉血亚硝酸盐／硝酸盐离子水平，并监测两组动物的血液动力学指标。结果发现：正常鼠肝脏的一氧化氮合成酶染色阴性，肝硬变鼠肝脏的再生假小叶内肝细胞的一氧化氮会成酶染色为强阳性；两组动物门静脉血的亚硝酸盐／硝酸盐离子的浓度分别为９．８±３．２ｕｍｏｌ／Ｌ，２６．７±６．８ｕｍｏｌ／Ｌ，差异有极显著性意义（Ｐ＜０．０１）；肝硬变大鼠的门静脉压力、门静脉血流量显著高于正常大鼠（Ｐ＜０．０５），而内脏血管阻力显著低于正常大鼠（Ｐ＜０．０５）。结果提示：一氧化氮在肝细胞的损伤及肝硬变门静脉高压的血液动力学改变中起重要作用。

The distribution of nitric oxide synthase (NOS) in the liver of normal and cirrhotic rats was ob- served and the levels of nitrits (NO-2) and nitrate (NO- 3) in portal vein and hemodynamic index were mea- sured.The results showed that:1.NOS in the liver of normal rat was negative.However,NOS in the pseu- dolobuli of cirrhotic rats was strong positive; 2. Com- pared with normal rats,the concentrations of NO-2 and NO-3 in the portal vein of cirrhotic rats were markedly elevated (P<0.01);3.Portal venous pressure and por- tal venous flow in cirrhotic rats were increased sihgifi- cantly (P<0.05),whereas splanchnic vascular resistance was decreased sihnficantly (P<0.05).It is suggested that nitric oxide may play an important role in the hepa- tocyte damge and the changes of hemodynamics in por- tal hypertension.