摘要
目的:探讨降钙素(CT)基因高甲基化能否作为骨髓增生异常综合征(MDS)向白血病转化的分子标志。方法:采用设有内、外参照的多聚酶链反应(PCR),结合限制性内切酶和激光扫描技术,检测27例MDS和6例由MDS转化的急性髓细胞白血病(AML)患者骨髓细胞的CT基因5′端甲基化率(CTMR)。结果:MDS患者CTMR为33.65%±23.37%,显著高于对照组(8.01%±4.25%,P<0.001),其中RAEBt组显著高于对照组和RA组,已随访3~10年的RA患者5例均在正常范围,且无白血病转化。9例RAEBt的CTMR均>30%,随访8例中7例于2个月内转化为AML。结论:CTMR对早期预测MDS向白血病转化可能是一个有用的指标。
Objective:To investigate the role of calcitonin gene hypermethylation in the transformation from myelodysplastic syndromes(MDS) to acute myeloid leukemias(AML). Methods:The methylation rate of calcitonin gene (CTMR) in the genomic DNA extracted from bone marrow cell of 27 MDS patients and 6 AML patients with antecedent MDS were studied by PCR amplification of Hpa Ⅱ digested DNA, with external reference of undigested and Msp Ⅰ digested DNA and internal reference of 112bp fragment containing N ras 61. Intensity of silver stained PCR products was measured by densitometer and analysed using the computer program. Results: The CTMR was significantly higher in MDS (33 65%±23.37%)than in control group(P<0.001),in 9 RAEB(T) (58.35%±17.44%) than in the control and RA groups.Five RA patients who have survived three to ten years without leukemia,have a normal CTMR.Seven of 8 RAEB(T) patients who had a high CTMR of more than 30% evolved into AML in two months. Conclusion: CTMR might be a useful marker for predicting the evolution of MDS into AML.
出处
《中华血液学杂志》
CAS
CSCD
北大核心
1997年第1期17-20,共4页
Chinese Journal of Hematology
基金
卫生部科研基金
CMB奖学金资助