白细胞介素-13基因多态性与哮喘易感的关系

Relationship between IL-13 Exon 4 Gene Polymorphisms A2 044G and the Patients with Asthma

目的:探讨白细胞介素(IL)-13外显子4单核苷酸多态性A2 044G基因与本地区人群哮喘易感性的相关性。方法:采用聚合酶链反应技术(PCR)对36例有明显支气管哮喘家族史的患者和20名健康对照者的IL-13 exon 4基因多态性进行检测,并进行序列测定。结果:正常对照组IL-13 exon 4第2044等位基因为A的频率是18%,为G是82%,支气管哮喘家族史的患者IL-13 exon 4第2044等位基因为A的频率是24%,为G是76%,与测序结果相同,经统计学分析,支气管哮喘组和正常对照组的等位基因频率差异具有统计学意义P<0.05。对照组GG、GA、AA基因型频率分别为66.1%、31.7%和2.1%;哮喘组GG、GA、AA基因型频率分别为60.0%、32.0%和8.0%。结论:IL-13+2044位A/G多态性与支气管哮喘的发病密切相关。

Objective：To study the role of the IL- 13 gene exon 4 A2 044G single nucleiofide polymorphism in the pathogenesis of bronchial asthma. Methods：The IL- 13 exon 4 was amplified by PCR genomic DNA used as templates from 20 healthy persons and 36 patients with dominantly allergic family history living in the local, The PCR products which were sequenced in the end. Results：There was significant difference in the distribution of A/G in IL- 13 exon 2 044, A allele frequency was higher in asthma compared with normal controls P〈0. 05, same as sequencing. GG、GA、AA genotype frequency were 66. 1%、31.7% and 2.1% in controls, respectively; GG、GA、AA genotype frequency were 60.0%、32.0% and 8.0% in asthma, respectively. Conclusion： IL- 13 A2 044G single nucleiotide polymorphism is important in asthmatic mechanism.