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AzaC预处理增加TCDD对特殊细胞P450基因的诱导 被引量：1

Enhancement of CYP450 Gene Expression of TCDD-inducible Specific Cells by AzaC Pretreatment
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摘要 利用RT-PCR检测不同物质诱导细胞的CYP基因 mRNA的表达水平.在HepG2细胞,TCDD能诱导CYP1A1、CYP1B1及CYP1A2基因表达,CYP1A1、CYP1B1基因比CYP1A2基因更容易被诱导,用AzaC处理后CYP1B1基因表达无改变;在A549细胞和SPC-A1细胞,Azac预处理后增加了TCDD对CYP1家族的诱导.也就是AzaC增加了CYP1A1、CYP1A2和CYP1B1基因表达水平. The expression levels of mRNA and protein of CYP450 genes were detected by reverse transcription - polymerase chain reaction （RT- PCR）. Human CYP1A1, CYP1B1 and mRNAs were constitutively expressed in HepG2 cells and were extensively induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin （TCDD）, but CYP1A2 was induced at low level. With AzaC, CYP1 B1 mRNA expression was not activated. In A549 cells and SPC-A1 cells, AzaC pretreatments increased the induction level of the CYP1 family by TCDD. AzaC treatment increased the constitutive expression levels of CYP1A1, CYP1A2 and CYP1B1. Fig 1, Tab 1, Ref 10
作者 刘燕群 谭佑铭 陈福生 周宜开
机构地区 江汉大学医学与生命科学学院 华中农业大学食品科学技术学院武汉 上海交通大学医学院 华中农业大学食品科学技术学院 华中科技大学同济医学院环医所
出处 《应用与环境生物学报》 CAS CSCD 北大核心 2007年第3期382-385,共4页 Chinese Journal of Applied and Environmental Biology
基金 国家自然基金资助项目(No.20275012) 湖北省自然科学基金资助项目(2006ABA166)~~
关键词 二恶英 人肺癌细胞 人肝癌细胞 P450基因 TCDD SPC-A1 cells A549 cells HepG2 cells CYP450 genes
分类号 X13 [环境科学与工程—环境科学]
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参考文献10

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同被引文献21

  • 1杨永滨,郑明辉,刘征涛.二恶英类毒理学研究新进展[J].生态毒理学报,2006,1(2):105-115. 被引量：47
  • 2毕恣,孙善全.水通道蛋白与甘油代谢[J].重庆医科大学学报,2007,32(9):1002-1004. 被引量：1
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  • 7Enan E, Lasley B, Stewart D, et al. 2,3,7,8-tetrachlorod- ibenzo-p-dioxin (TCDD) modulates function of humanluteinizing granulosa cells via cAMP signaling and ear- ly reduction of glucose transporting activity [J]. Repro- ductive Toxicology, 1996, 10(3): 191 - 198.
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应用与环境生物学报
2007年 第3期

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