匍扇藻多糖抗病毒活性的初步研究(英文)

THE ANTIVIRAL ACTIVITY OF POLYSACCHARIDES EXTRACTED FROM LOBOPHORA VARIEGATA

目的:研究褐藻匍扇藻(Lobophora variegata)多糖的抗病毒活性。方法:匍扇藻多糖的粗提物经沸水提取和乙醇沉淀后获得,粗多糖经离子交换柱层析法分离以获取较纯的多糖片段。它们的抗病毒活性采用细胞病变效应减少法和空斑减少检测法检测。粗多糖的细胞毒性采用 MTT 法测定。结果:匍扇藻粗多糖具有显著的抗单纯疱疹病毒Ⅰ型和Ⅱ型的活性,它能抑制不同的单纯疱疹病毒毒株,包括标准株、阿昔洛韦抗性株和临床病毒株。通过空斑减少法测得它对阿昔洛韦抗性株和 HSV-Ⅱ型临床株的半数有效浓度(EC50)分别为 18.2 和 6.25 μg/ml。因为它的细胞毒性很低,因此具有较大的选择性系数。这种粗多糖有一定的抗呼吸道合胞病毒(RSV)活性,但对流感病毒没有抑制作用。该粗多糖经DEAE离子交换柱层析分离得到两个不同的多糖,它们也都显示出抗单纯疱疹病毒的活性。结论:匍扇藻含有具抗病毒活性的多糖并且对单纯疱疹病毒显示出较强的抑制作用。

Objective： To study the antiviral effect of polysaccharides from brown seaweed Lobophora variegata. Method： The crude polysaccharide was extracted with boiling water and precipitated with ethanol, and then fractionated with ion exchange chromatography. Its antiviral activity was tested by cytopathic effect （CPE） reduction assay and plaque reduction assay. The cytotoxicity of the water crude extract was determined by MTT method. Results： The crude water extract showed markedly antiviral activity against herpes simplex virus （HSV） type 1 and type 2 including ACV resistant strain and clinical strains with low ECs0 values of 18.2 and 6.25 μg/ml respectively. It also showed very low cytotoxicity to Veto, HEp-2 and MDCK cells. Therefore, the crude extract possessed high selective index for antiviral effect. It also had some inhibitory effect on respiratory syncytial virus （RSV） but had no effect on influenza A virus. Two fractions were isolated from the water crude extract and they exhibited anti-HSV activity in the test of CPE reduction assay in Vero cells. Conclusion： Seaweed Lobophora variegata contains antiviral polysaccharides with high inhibitory effect on herpes simplex virus.