期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

葡多酚对乳腺癌细胞P38MAPK信号传导通路的影响

THE INHIBITORY EFFECT OF GRAPE PROANTHOCYANIDINS ON P38MAPK PATHWAY IN BREAST CANCER CELLS
下载PDF
导出
摘要 目的:探讨葡多酚(grape proanthocyanidins,GPC)对P38MAPK(P38 mitogen-activated protein kinase,P38 MAPK)信号转导通路的影响。方法:将接种EMT-6乳腺癌细胞的BALB/C小鼠分别经口灌胃给予10、100、200mg/kg bw GPC,连续2w。用Western blot方法检测肿瘤组织磷酸化P38MAPK蛋白和MMP-2蛋白的表达水平。结果:肿瘤对照组和200mg/kgGPC组的磷酸化P38MAPK蛋白表达水平(相对灰度值)分别为1.16±0.18和0.58±0.12,MMP-2蛋白表达水平分别为0.98±0.04和0.69±0.04,差别均有显著性意义(P<0.05)。结论:GPC对乳腺癌P38MAPK信号转导通路的活化和MMP-2蛋白的表达均有一定抑制作用。 Objective： To investigate the inhibitory effect of grape proanthocyanidins （GPC） on P38 mitogen-activated protein kinase （P38MAPK） pathway in mice with breast cancer, Methods： BALB/C mice inoculated with EMT-6 breast cancer cells were given 10, 100 and 200 mg/kg bw GPC po daily for 2 w, The expression levels of phosphorylated P38MAPK and matrix metalloproteinase-2 （MMP-2） protein were detected by Western blot, Results： The levels of phosphorylation P38MAPK in tumor control group and 200 mg/kg GPC group were 1.16±0.18和0.58±0.12 respectively, and the levels of MMP-2 protein were 0.98±0.04和0.69±0.04 respectively. The differences between the two groups were significant （P〈0,05）. Conclusion： GPC can inhibit P38MAPK pathway and the expression of MMP-2 protein in breast cancer cells.
作者 赵蓉 钟进义
机构地区 青岛大学医学院
出处 《营养学报》 CAS CSCD 北大核心 2007年第3期295-297,共3页 Acta Nutrimenta Sinica
基金 国家自然科学基金(No.30471457)
关键词 葡多酚 乳腺癌 P38MAPK 基质金属蛋白酶-2 信号传导 grape proanthocyanidins breast cancer P38MAPK matrix metalloproteinase-2 signal pathway
分类号 R737.9 [医药卫生—肿瘤]
  • 相关文献

参考文献11

  • 1曾亮,曹亚.MAPK信号通路与肿瘤侵袭和转移研究进展[J].肿瘤防治研究,2002,29(5):419-421. 被引量：12
  • 2唐皓,马中富.p38 MAPK信号转导通路研究进展[J].热带医学杂志,2004,4(4):501-504. 被引量：15
  • 3Huang X,Chen S,Xu L,et al.Genistein inhibits P38 MAP kinase activation,matrix metalloproteinase type 2,and cell invasion in human prostate epithelial cells[J].Cancer Res,2005,65:3470-3478.
  • 4Ray SD,Parikh H,Bagchi D.Proanthocyanidin exposure to B6C3F1 mice significantly attenuates dimethylnitrosamine-induced liver tumor induction and mortality by differentially modulating programmed and unprogrammed cell deaths[J].Mutat Res,2005,579:81-106.
  • 5Mendes O,Kim HT,Stoica G.Expression of MMP-2,MMP-9 and MMP-3 in breast cancer brain metastasis in a rat model[J].Clin Exp Met,2005,22:237-246.
  • 6Lochter A,Srebrow A,Sympson CJ,et al.Misregulation of stromelysin-1 expression in mouse mammary tumor cells accompanies acquisition of stromelysin-1 dependent invasive properties[J].J Biol Chem,1997,272:5007-5015.
  • 7Bradham C,McClay DR.P38MAPK in Development and Cancer[R].Cell Cycle,2006,5:824-828.
  • 8Huang S,New L,Pan Z,et al.Urokinase plasminogen activator/urokinase-secific surface receptor expression and matrix invasion by breast cancer cells requires constitutive P38 alpha mitogen-activated protein kinase activity[J].J Biol Chem,2000,275:12 266-12 272.
  • 9Simon C,Goepfert H,Boyd D.Inhibition of the P38 mitogen-activated protein kinase by SB 203580 blocks PMA-inducedMr92,000 type Ⅳ collagenase secretion and in vitro invasion[J].Cancer Res,1998,58:1135-1139.
  • 10Praveen K,Anshu M,Stantosh K,et al.Proanthocyanidins from grape seeds inhibit expression of matrix metalloproteinases in human prostate carcinoma cells,which is associated with the inhibition of activatior of MAPK and NF-KB[J].Oxford J,2004,25:987-995.

二级参考文献60

  • 1国植,徐莉.原花青素:具有广阔发展前景的植物药[J].国外医药（植物药分册）,1996,11(5):196-204. 被引量：178
  • 2[1]Jukka W, Veli-Matti K. Regulation of matrix metalloproteinase expression in tumor invasion. FASEB J, 1999, 13:781-792.
  • 3[2]Johansson N, Ala-aho R, Uitto V, et al. Expression of collagenase-3(MMP-13) and collagenase-1(MMP-1) by transformed keratinocytes is dependent on the activity of p38 mitogen-activated protein kinase. J Cell Sci , 2000, 113 pt 2:227-235.
  • 4[3]Simon C, Hicks MJ, Nemechek AJ, et al. PD098059, an inhibitor of ERK1 activation, attenuates the in vivo invasiveness of head and neck squamous cell carcinoma. Br J Cancer, 1999, 80(9):1412-1419.
  • 5[4]Vo HP, Lee MK, Crowe DL.alpha2beta1 integrin signaling via the mitogen activated protein kinase pathway modulate retinoic acid-dependent tumor cell invasion and transcriptional downregulation of matrix metalloproteinase 9 activity . Int J Oncol, 1998, 13(6):1127-1134.
  • 6[5]Tsang KJ, Crowe DL. Retinoic acid and extracellular matrix metalloproteinase 9 expression is mediated by the mitogen activated protein kinase pathway. Int J Oncol, 2001, 18(2):369-374.
  • 7[6]Bancroft CC, Chen Z, Dong G, et al. Coexpression of proangiogenic factors IL-8 and VEGF by human head and neck squamous cell carcinoma involves coactivation by MEK-MAPK and IKK-NF-kappaB signal pathways. Clin Cancer Res, 2001,7(2):435-442.
  • 8[7]Yoshiji H, Kuriyama S, Ways DK, et al. Protein kinase C on the signaling pathway for vascular endothelial growth factor-mediated tumor development and angiogenesis. Cancer Res, 1999, 59(17):4413-4418.
  • 9[8]Okajima E, Thorgeirsson UP. Different regulation of vascular endothelial growth factor expression by the ERK and p38 kinase pathways in V-ras, v-raf, and v-myc transformed cells. Biochem Biophys Res Commun, 2000, 270(1):108-111.
  • 10[9]Harris VK, Coticchia CM, Kagan BL, et al. Induction of the angiogenic modulator fibroblast growth factor-binding protein by epidermal growth factor is mediated through both MEK/ERK and p38 signal transduction pathways. J Biol Chem, 2000, 275(15):10802-10811.

共引文献34

营养学报
2007年 第3期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部