大蒜素油对三丁基锡引起的小鼠氧化损伤的保护作用

Protective Effects of Garlic Oil on Tributyltin-induced Oxidative Damage in Mice

采用灌胃的方法,对雄性小鼠进行三丁基锡(TBT)染毒,剂量为1mg/(kg.d),然后分别用不同剂量的大蒜素油进行保护。保护组高剂量为200mg/(kg.d),低剂量为100mg/(kg.d),试验7d。试验结果表明:大蒜素油保护组小鼠肝组织活性氧(ROS)活性和丙二醛(MDA)含量均明显低于TBT对照组;电镜观察发现TBT染毒后小鼠胸腺细胞核损伤较为明显,主要是染色质高度凝聚、边缘化,甚至崩解消失,而大蒜素油保护组细胞核损伤明显减轻;彗星试验发现TBT染毒组淋巴细胞尾长和尾相都显著升高,而大蒜素油保护组尾长和尾相与TBT对照组差别明显,并与剂量相关。因此小鼠TBT染毒后给予一定剂量的干预剂——大蒜素油,对TBT引起的细胞核损伤有明显的保护作用。

In present study, male mice were pretreated with Tributyltin at the dosage of lmg/（kg·d）, and then gavaged with garlic oil at different dosages [200 mg/（kg·d） and 100 mg/（kg·d）] as protective strategy. Mice were sacrificed after 7 days treatment. Livers were obtained for measuring the level of reactive oxygen species （ROS） and contents of malondialdehyde （MI）A） . Thymus and blood were obtained for pathologic observation and single-cell gel-electrophoresis （comet assay）, respectively. Results showed that ROS production and contents of MDA decreased significantly in garlic oil-protected groups compared with TBT control group. Under electron microscope observation, damage of nucleoli can be found in non-protected group presenting the agglomerate and fringing of chromatin. The results suggest that garlic oil can prevent DNA damage induced by TBT induced oxidative stress. While the damages of nucleolus of thymocytes were reduced in a dose-dependent manner in garlic oil protected groups, in comet assay, tail length and tail moment of mice lymphocyte in garlic oil protected groups were also reduced and obversely different with TBT control group in a dose-dependent manner. The results suggest that garlic oil has convinced prgtective effects on the damage of nucleolus induced by TBT.