桑黄菌液体发酵培养基研究

Studies on the Culture Medium for Liquid Fermentation of Phellinus Igniarius

目的:探讨桑黄菌液体发酵的适宜培养基,为进一步研究其液体发酵工艺及提取有效成分做准备。方法:首先用单因素单指标法筛选,再用正交试验双指标法筛选。结果:桑黄菌生长所需最适C/N比为24;最适的碳源为黄豆粉和玉米粉,适宜的含量分别为4%和3%;最适的氮源为小麦麸粉,适宜的含量为0.5%;对菌丝生长发育影响显著的物质有FeSO4、ZnSO4、K2SO4、CaSO4、Na2HPO4、VB1等。结论:桑黄菌液体发酵适宜的培养基配方(g/L):黄豆粉40、玉米粉30、小麦麸粉5、酵母浸出汁4、CaSO410、K2SO41、Na2HPO41.5、ZnSO4.7H2O0.1、Fe-SO4.7H2O0.5、VB10.1。

Objective： To explore the suitable culture mediums for liquid fermentation of Phellinus igniarius and supply reference for further study its liquid ferment technology and extract its available components. Methods： In the first instance, it was studied by the method of single factor and single target, followed by the method of orthogonal experiment with double targets. Results： The most suitable ratio of C/N for growth of Phellinus ignian＇us was 24; The most suitable carbon source was soybean powder and corn powder and its suitable concentration were 4% and 3% respectively; The most suitable nitrogen source was wheat bran powder and its suitable concentration was 0.5%; The inorganic salts and vitamin B, which have prominent effect on the mycelium development, including FeSO4. ZnSO4. K3SO4. CaSO4, Na2HPO4, VB1 and so on. Conclusion： The suitable culture medium for liquid fermentation of Phellinus igniatius was as follow （g/L）： Soybean powder 40, corn powder 30, wheat bran powder 5, the yeast juice 4, CaSO4 10, K2SO4 1, Na2HPO4 1.5, ZnSO4·7H2O 0.1, FeSO4·7H2O 0.5, VB1 0.1.