4~5周龄D_2BF1小鼠的血液学参考值、主要脏器重量及脏器系数的测定

The Measurement of Hematological Parameter,Main Organ Weight and Organ Coefficient in D_2BF1 Mice

目的测定4~5周龄D2BF1小鼠血液学参考值,且与同龄DBA/2、C57BL小鼠比较,并提供D2BF1小鼠主要脏器重量、脏器系数的参考值。方法随机选取4~5周龄D2BF1小鼠雌雄各10只,分别称重,摘除眼球采血后,颈椎脱臼处死,摘除主要脏器称重,并将测定的血液学值与同龄的C57BL和DBA/2小鼠进行比较分析。结果D2BF1小鼠雌雄之间血液值MCV有显著性差异(P<0.05),其余无显著性差异(P>0.05)。与同龄的C57BL小鼠相比,血液值WBC、Lymph%、Gran%有极显著性差异(P<0.01)。其余无显著性差异(P>0.05)。与同龄的DBA/2小鼠相比,WBC、Mid%、RBC、MCH有极显著性差异(P<0.01),Mid有显著性差异(P<0.05)。其余无显著性差异(P>0.05)。结论D2BF1代小鼠白细胞及分类明显低于C57BL和DBA/2小鼠,值得进一步研究。

Objective To measure 4 - 5 weeks D2BF1 mice＇ s hematological parameter and compare the inbred strain C57BL and DBA/2, and provide D2 BF1 mice＇ s main organ weight and organ coefficient. Methods 4 - 5 weeks mice were chosen at random for this experiments. Result and Conclusion The values of hematology＂ were not significant differences between male and female D2 BF1 except for the value of MCV. WBC, Lymph% and Gran% of D2 BF1 had significant differet^ces compared with that of C57BL. Meanwhile, WBC, Mid%, RBC and MCH also had significant differences compared with that of DBA/2.