IL-18基因佐剂协同HSV-1 gB DNA疫苗免疫诱导的特异性免疫应答

Enhancement of HSV-1 glycoprotein-B specific immunity induced by DNA vaccine combined with IL-18 genetic adjuvant

目的研究IL-18cDNA协同单纯疱疹病毒Ⅰ型(HSV-1)糖蛋白B(gB)核酸疫苗免疫对机体体液免疫和细胞免疫应答的影响。方法利用pcDNA3载体分别构建HSV-1gB和IL-18的真核表达质粒pgB和pIL-18,分pcDNA3、pgB和pgB+pIL-18三组,肌肉注射免疫接种BALB/c小鼠3次,每次间隔2周,每次接种质粒100μg,第3次免疫后2周,用ELISA检测特异性抗体滴度;利用3H-TdR掺入法进行T细胞特异性抗原刺激实验;耳廓肿胀实验检测迟发型超敏(DTH)反应。结果与pgB单独免疫组相比,pIL-18的协同免疫可以显著增强ELISA特异性抗体滴度、T细胞增殖反应和DTH反应。结论IL-18cD-NA的协同免疫可以显著提高pgBDNA疫苗诱导的体液免疫和细胞免疫水平,增强核酸疫苗对机体的免疫保护作用。

Objective：To investigate the efficacy of IL-18 as a genetic adjuvant for DNA vaccine-induced immune responses. Methods：BALB/e mice were immunized by three intramuscular inoculations of HSV-1 glycoprotein B（gB） DNA vaccine alone or in combination with the plasmid expressing mature IL-18 peptide, at the interal of 2 weeks. After three weeks immunization, antibody titers in the sera were determined by ELISA, and splenic cell proliferation was detected with s H-TdR incorporation assay. Delayed type hypersensitivity（DTH） response was detected by the pinna-swelling test. Results：Compared with injection of gB DNA alone, IL-18 plasmid co-injection significantly increased antibody titers, and enhanced antigen-specific lymphocyte proliferation and efficiently the delayed-type hypersensitivity response. Conclusion： IL-18 eDNA can enhance both the humoral and cellular immune responses.