摘要
以印度辣木栽培品种PKM(Moringa oleifera PKM-1)一年生植株茎尖为外植体,采用秋水仙素结合组织培养技术诱导多倍体的方法,研究了秋水仙素浓度及处理时间对诱导染色体倍性变异的影响,以及不同外源植物生长调节剂浓度对茎段培养的增殖影响,结果表明:辣木芽初代培养的最适培养基为MS+6-BA2.0mg/L+NAA0.2mg/L,不定芽增殖的最佳培养基为MS+6-BA0.6mg/L,增殖系数为3.57,用0.1%秋水仙素处理24h左右,可诱导获得四倍体再生植株。
A study of inducing polyploidy by treating in vitro stem segment of Moringa oleifera, PKM-1, was carded out with different concentrations of colchicines solution and different treated durations, also the effect of different exterior plant growth regulators with different concentration on proliferation.The results indicated that MS＋6-BA2.0 mg/L＋NAA0.2 mg/L was the optimum medium for generation, MS＋6- BA 0.6 mg/L was the favorable medium for adventitious bud proliferate medium, tetraploid plantlets were obtained by treatment with 0.1% colchicines for about 24h.
出处
《热带农业科技》
2007年第1期27-30,共4页
Tropical Agricultural Science & Technology
基金
农业部948后续项目(2005)
关键词
辣木
组织培养
秋水仙素
四倍体
Moringa oleifera
tissue culture
colchicines, tetraploid
