宁波地区大肠埃希菌和肺炎克雷伯菌质粒AmpC酶基因检测

Detection of plasmid-mediated AmpC β-lactamases genes in escherichia coli and klebsiella pneumoniae in Ningbo area

目的了解近三年宁波地区大肠埃希菌和肺炎克雷伯菌临床分离株中质粒AmpC酶基因流行状态。方法用K-B法对临床分离的大肠埃希菌和肺炎克雷伯菌作耐药表型分析,对可能产生Am-pC酶的菌株通过聚合酶链反应(PCR)检测AmpC酶基因,然后用三维试验予以证实。结果140株大肠埃希菌和肺炎克雷伯菌中,质粒AmpC酶基因阳性率2004、2005和2006年分别为4.4%(2/45)、8.6%(3/35)和13.3%(8/60);其中2004、2005年所检出的均为DHA基因型,2006年分别从2株大肠埃希菌和1株肺炎克雷伯菌中检出ACT-1基因。结论宁波地区临床分离的大肠埃希菌和肺炎克雷伯菌中质粒AmpC酶基因的流行率有增长趋势。

Objective To investigate the prevalence and genotype of plasmid-mediated AmpC β-lactamases genes in Escherichia coli and Klebsiella pneumoniae strains isolated from Ningbo area. Methods After the detection for drug resistant phenotypes by K-B method, positive strains were subjected by polymerase chain reaction （PCR）, then qualified through three-dimension test. Results From year 2004 to 2006, the positive rates of plasmid-mediated AmpC genes were 4.4% （2/45）, 8.6% （3/35）, 13.3% （8/60）, respectively;Meanwhile, the unique genotype was DHA for all positive strains in years 2004 and 2005, As for 2006, ACT-1 genotype has appeared in three strains. Conclusions Though plasmid mediated AmpC genes in Ningbo area is not quite prevalent in Escherichia coIi and Klebsiella pneumoniae, there is an increasing tendency in recent years.