碱性纤维素酶发酵工艺的初步研究

Fermentation technology of alkaline cellulase

从碱性土样中分离出能产生胞外碱性纤维素酶的耐碱菌株A1,初步鉴定为芽孢杆菌属。经条件试验后,该菌产生碱性纤维素酶的最适培养温度为37℃,最适碳源为淀粉,最适氮源为牛肉膏,添加0.5%-1.0%NaCl和0.2%左右的Na2CO3时可以提高产酶量,在适宜的产酶条件下酶活可以达到42.5 U/ml。

The strain A1 isolated from an alkaline soil could produce alkaline cellulase, and was identified as Bacillus sp. The results showed that the optimal fermentation conditions of producing alkaline cellulase by the strain A1 were as follows： optimal temperature was 37 ℃, optimal carbon resource was starch, optimal nitrogen resource was beef extract. Addition of 0.5% - 1.0% NaCl and 0.2 % Na2CO3 could increase alkaline cellulase enzyme activity of strain A1 .The activity of alkaline cellulase was 42.5 U/ml at the suitable condition of producing alkaline cellulase.