早、中、晚孕期胎盘因子对人外周血淋巴细胞CD4、CCR5和CXCR4表达的影响

Effects of the 1^(st),2^(nd),and 3^(rd) trimester placental factors on CD4,CCR5,and CXCR4 expression on human peripheral blood lymphocytes

目的通过研究早、中、晚孕期胎盘因子(PF)对人外周血淋巴细胞(PBLs)中CD4、CCR5和CXCR4表达的作用,探讨PF在人免疫缺陷病-毒1(HIV-1)垂直传播中的作用及其机理。方法制备早、中、晚孕期PF。分离人外周血单个核细胞,并分别与相对浓度为25%的早、中、晚孕期PF作用,培养24 h后收集细胞,荧光抗体标记,流式细胞术检测外周血淋巴细胞(PBLs)中CD4、CCR5和CXCR4表达,以及CD4+T细胞中CCR5+细胞、CXCR4+细胞、CCR5+CXCR4+细胞所占的百分率。结果各孕期PF均可显著降低PBLs中CCR5的表达,其中早孕期PF的作用明显强于中、晚孕期PF的作用;各孕期PF组CD4+T细胞中CCR5+细胞的百分率均显著低于对照组,早孕期PF组CD4+T细胞中CCR5+细胞的百分率明显低于中、晚孕期PF组;各孕期PF组CD4+T细胞中CCR5+CXCR4+细胞的百分率均显著低于对照组,早孕期PF组CD4+T细胞中CCR5+CX-CR4+细胞的百分率显著低于晚孕期PF组。结论各孕期PF均可显著降低PBLs中CCR5的表达,以及CD4+T细胞中CCR5+细胞和CCR5+CXCR4+细胞的百分率,早孕期PF作用最强,中、晚孕期PF效应相当,PF可能通过抑制R5病毒的入胞而具有抗R5病毒的作用,并可能在阻断HIV-1宫内感染中具有重要作用。

Objective To investigate the effects of the 1^st, 2^nd, and 3^rd trimester placental factors （PFs） on CD4, CCRS, and CX- CR4 expression on humnan peripheral blood lymphocytes （PBLs）, and to explore the effects of PFs on human immunodeficiency virus type 1 （HIV-1） vertical transmission and their possible anti-HIV-1 mechanisms. Methods Human peripheral blood mononuclear cells were treated with the 1^st, 2^nd, and 3^rd trimester PF （concentration 25% ） respectively for 24 h. The CD4, CCRS, and CXCR4 expressions on PBLs as well as the percentages of CCR5^＋ cells, CXCR4 ^＋ cells, and CCR5 ^＋ CXCR4 ^＋ cells in CD4 ^＋ cells were detected by flow cytometry. Results All trimester PFs reduced the CCR5 expression on PBLs. The efficiency of the 1^st trimester PF was higher than those of the 2^nd and 3^rd trimester PFs. The percentage of CCR5 ^＋ cells in CD4^＋ T cells of PF groups was significantly lower when compared with the control group; the percentage of CCR5 ^＋ cells in CD4^＋ T cells of the 1^st trimester PF group was significantly lower than those of the 2^nd and 3^rd trimester groups. The percentage of CCR5^＋ CXCR4 ^＋ cells in CD4 ^＋ T cells of PF groups was significantly lower when compared with the control group; the percentage of CCR5^＋ CXCR4 ^＋ cells in CD4 ^＋ T cells of the 1^st trimester PF group was significantly lower than that of the 3^rd trimester PF group. Conclusion PF can reduce the expression of CCR5 on human PBLs and CD4^＋ T cells. PF might reduce R5 virus infection via preventing HIV-1 entry, and might play an important role in reducing R5 virus intrauterine infection.