期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

荧光显微镜观察银杏内酯B对大鼠心肌细胞凋亡的影响 被引量：4

Effects of Ginkgolide B on the Apoptosis in Rat Cardiomyocytes by Fluorescent Microscope
下载PDF
导出
摘要 目的:建立一种简便、快速的细胞凋亡检测方法,探讨银杏内酯B(GB)对心肌细胞凋亡的影响。方法:体外培养大鼠心肌细胞,利用荧光显微镜观察经Hoechst33258染色的心肌细胞,记录凋亡细胞核形态学改变并计算凋亡百分率。结果:H2O2可诱导心肌细胞发生凋亡,与H2O2组相比,GB组大鼠心肌细胞凋亡率明显降低,并有显著性差异(P<0.05)。结论:银杏内酯B对大鼠心肌细胞凋亡具有保护作用。 Objective： A method was developed to determine the apoptosis rapidly. We observed the effect of Ginkgolide B （GB） on the apoptosis in rat cardiomyocytes. Methods： Rats cardiomyocytes were cultured in vitro. Apoptotic morphological changes and percentage of apoptosis were assayed with Hoechst 33258 staining and were observed under fluorescent microscope. Results ： H2O2 caused apoptosis on the cultured rat cardiomyocytes. After treatment with GB, rat cardiomyocytes apoptosis decreased significantly （ P 〈 0.05 ）. Conclusions： GB might exert protective effects on rat cardiomyocytes against apoptosis.
作者 于力方 廖杰 王珊 李宁 梅世昌
机构地区 解放军总医院医学实验测试中心
出处 《现代科学仪器》 2007年第3期70-72,共3页 Modern Scientific Instruments
关键词 细胞凋亡 心肌细胞 银杏内酯B Cell Apoptosis cardiomyocytes ginkgolide B
分类号 R285.5 [医药卫生—中药学]
  • 相关文献

参考文献7

二级参考文献20

  • 1[1]Suzuki S,Kostin S,Person V,et al. Time course of the apoptotic cascade and effects of caspase inhibitors in adult rat ventricular cardiomyocytes[J]. J Mol Cell Cardiol, 2001,33:983-994.
  • 2[2]Semenovsky ML, Shumakov VI, Sharov VG, et al. Protection of ischemic myocardium by exogenous phosphocreatine[J]. J Thorac Cardiovasc Surg, 1984,87:190-200.
  • 3[3]Glick R,Burns H, Reddy J, et al. Dispersion and isolation of beating cells from adult rat heart[J]. Anal Biochem,1974,61:32-42.
  • 4[4]Halestrap AP, Kerr PM, Javadov S, et al. Elucidating the molecular mechanism of the permeability transition pore and its role in reperfusion injury of the heart[J]. Biophys Acta, 1998,1366:79-94.
  • 5[5]Mathur A, Hong Y,Kemp BK, et al. Evaluation of fluorescent dyes for the detection of mitochondrial membrane potential changes in cultured cardiomyocytes[J]. Cardiovasc Res, 2000, 46: 126-138.
  • 6[6]Down WH, Chasseaud LF, Ballard SA, et al. The effect of intravenously administered phosphocreatine on ATP and phosphocreatine concentrations in the cardiac muscle of the rat[J]. Drug Res,1983, 33(I):552-554.
  • 7[7]Jacobus WE. Respiratory control and the integration of heart high-energy phosphate metablism by mitochondrial creatine kinase[J]. Ann Rev Physiol, 1985,47:707-725.
  • 8Li S, Meng Q, Zhang L. Experimental therapy of a platelet activating factor antagonist (ginkgolige B) on photochemically induced thrombotic cerebral ischemia in tree shrews [J]. Clin Exp Pharmacol Physiol, 1999,26 : 824 - 825.
  • 9Kornecki E,Ehrich H Y. Neuroregulatory and neuropathological actions of the ether -phospholipid platelet -activating factor[J]. Science, 1988,4860 : 1792 - 1794.
  • 10Nishida K, Markey S P. Platelet - activating factor in brain regions after transient ischemia in gerbils [J]. Stroke, 1996,27 :514-518.

共引文献108

同被引文献43

引证文献4

二级引证文献23

现代科学仪器
2007年 第3期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部