温室白粉虱抗药性成虫粘胶皿测定法

Monitoring of Insecticide Resistance in Adult of Greenhouse Whitefly with Sticky Dishes

将不同浓度的杀虫药剂丙酮液与粘蝇胶按1：1（V／W）混合涂于直径45mm培养血的皿底上，粘着温室白粉虱成虫，测定其抗药性程度。结果表明，对照死亡率一般不超过5％，每种药剂经2～3次独立试验证明，结果稳定，溴氰菊酯、氰戊菊酯、乐果、氧乐果和马拉硫磷对番茄上白粉虱的责力（LC50）分别为23．726，197．439，0．895和0．446mg·g-1胶，与喷雾法进行了比较，毒性比值（TR），溴氰菊酯、氰戊菊酯、乐果、氧乐果和马拉硫磷分别为。004，0．0027，6．271，3．439和0．446。乐果、氧乐果与两个菊酯类药剂比值相差很大，可能与乐果，氧乐果具有内吸性和易溶于水，而菊酯类无内吸性和几乎不溶于水有关，优乐得可以用喷雾法监测其抗性，而粘胶法则不能，本文也对成虫喷雾法、黄粘胶卡法和粘胶皿法的优缺点进行了比较。

The use of 45 mm petri dishes which bottom was coated with a thin layer of in-secticide acetone solution mixed with an elastomers,fly sdhesive (1 mL .g-1sticker), for monitoring of insecticide resistance in adult greenhouse whitefly Trialeurodes vaporariorum (Westwood)was investigated. The mortality response of the adult of greenhouse whitefly,captured from greenhouse tomato by fly adhesive was istimated after 24 h under 25'C room temperature. The results showed that this technique provided a stable LC,,, with a low control mortality. The LC50 's of deltamethrin, fenvalerate, dimethoate, omethoate, and malathion were 23. 726, 197. 439, 0. 859, 0. 255, and 35. 640 mg .g-1sticker respectively.When compared with adu1t spray method for monitoring of imsecticide resistance, the toxi-city ratios (TR)of deltamethrin, fenvalerste, demethoate, omethoate,and malathion were 0.004, 0. 002 7, 6. 274, 3. 439, and 0.446 respectivly, this significant difference of TR be-tween pyrethroid and dimethoate or omethoatemay be related to the good systemic proper-ty and water solubility of dimethoate or ometnoate, while pyerthroid does not have sys-temic property and do have very low water solubility. For the insect growth regulator,buprofezin, resistance can not be estimated with adult sticky dishes method, but can be done with adult spray methbd. The advantages and disadvantages of the three methods for monitoring of insecticide resistance in adult whitefly were discussed.