期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

RNAi与肿瘤相关基因及在肿瘤治疗中的研究 被引量：1

原文传递
导出
摘要 RNA干扰（RNA interfcrence，RNAi），是一种在动植物中广泛存在的通过双链RNA分子在mRNA水平上诱导特异性序列基因沉默的过程，随着对RNAi研究的不断深入，其作用机制正在逐步被阐明；同时作为阻断基因表达的新手段，RNAi技术也日趋完善和成熟。而恶性肿瘤基因学说也越来越得到重视，肿瘤相关基因的研究更加细致深入。本文就RNAi技术的基本原理、肿瘤相关基因的概念以及RNAi技术在肿瘤尤其是泌尿系肿瘤相关基因研究中的应用作一综述。
作者 喻彬（综述） 陈炜（审校）
机构地区 广州中山大学第一附属医院泌尿外科
出处 《国际泌尿系统杂志》 2007年第4期489-494,共6页 International Journal of Urology and Nephrology
关键词 泌尿系肿瘤 癌基因 RNA
分类号 R737.1 [医药卫生—肿瘤]
  • 相关文献

参考文献25

  • 1Hammond SM , Bernstein E , Beach D , et al. An RNA - directed nuclease mediates post - transcriptional gene silencing in Drosophila-cells. Nature , 2000 , 404 (6775) : 293 -296.
  • 2Elbashir SM , Harborth J , Lendeckel W, et al. Duplexes of 21 - nuckeotide RNAs mediate RNA interference in cultured mammalian-cells. Nature ,2001 ,411 (6836) :494-498.
  • 3Bemstein E , Caudy AA , Hammond SM , et al. Role for a bidentate ribonuclease in the initiation step of RNA interference. Nature , 2001 ,409 (6818) : 363 -366.
  • 4Dalmay T , Hamilton A , Rudd S , et al. An RNA - dependent RNA polymerase gene in Arabidopsis is required for posttranscriptional gene silencing mediated by a transgene but not by a virus. Cell , 2000 , 101 (5) :543-553.
  • 5Elbashir SM , Lendeckel W, Tuschl T. RNA interference is mediated by 21-and22-nucleotide RNAs. Genes Dev , 2001 ,15 (2) : 188 -200.
  • 6Hannon GJ . RNA interference. Nature , 2002 ,418(6894) :244 - 251.
  • 7Tuschl T. RNA interference and small interference RNAs. Chem - biochem ,2001,2 (4) :239 - 245.
  • 8Ngo H , Tschudi C , Gull K, et al. Double - stranded RNA induces mRNA degradation in Trypanosoma brucei. Proc Natl Acad Sci USA , 1998 ,95 (25) :14687 - 14692.
  • 9Montgomery MK, Xu S , Fire A. RNA as a target of double - stranded RNA mediated genetic interference in Caenorhabditis elegans. Proc Natl Acad Sci USA,1998,95 (25) :15502 -15507.
  • 10Lipardi C , Wei Q , Paterson BM. RNAi as random degradative PCR : siRNA primers convert mRNA into dsRNAs that are degraded to generate new siRNAs. Cell ,2001,107 (3) :297 - 307.

二级参考文献22

  • 1Weiss JM, Nath A, Major EO, et al. HIV-1 Tat induces monocyte chemoattractant protein-l-mediated monocyte transmigration across a model of the human blood-brain barrier and up-regulates CCR5 expression on human monocytes[ J]. J Immunol, 1999,163 ( 5 ) :2953-2959.
  • 2Bowman T, Garcia R, Turkson J, et al. STATs in oncogenesis[J]. Oncogene, 2000, 19(21) :2474-2488.
  • 3Grandis JR, Drenning SD, Zeng Q, et al. Constitutive activation of STAT3 signaling abrogates apoptosis in squamous cell carcinogenesis in vivo [ J ]. Proc Natl Acad Sci USA, 2000, 97 (8):4227 -4232.
  • 4Schuringa JJ, Wierenga AT, Knfijer W, et al. Constitutive STAT3,Tyr705, and Ser727 phosphorylation in acute myeloid leukemia cells caused by the autocrine secretion of interleukin-6 [ J ].Blood, 2000, 95(12) :3765-3770.
  • 5Badache A, Hynes NE. Interleukin 6 inhibits proliferation and,in cooperation with an epidermal growth factor receptor autocrine loop, increases migration of T47D breast cancer cells [ J ]. CancerRes, 2001,61 ( 1 ) :383-391.
  • 6Buettner R, Mora LB, Jove R. Activated STAT signaling in human tumors provides novel molecular targets for therapeutic intervention[J]. Clin Cancer Res, 2002, 8(4) :945-954.
  • 7Bromberg JF, Horvath CM, Besser D, et al. STAT3 activation is required for cellular transformation by v-src [ J ]. Mol Cell Biol,1998, 18(5) :2553-2558.
  • 8Leong PL, Andrews GA, Johnson DE, et al. Targeted inhibition of STAT3 with a decoy oligonucleotide abrogates head and neck cancer cell growth[J]. Proc Natl Acad Sci USA, 2003,100(7) :4138-4143.
  • 9Huang JS, Guh JY, Hung WC, et al. Role of the Janus kinase(JAK)/signal transducers and activators of transcription (STAT)cascade in advanced glycation end-product-induced cellular mitogenesisNRK-49F cells [J]. Biochem J, 1999, 342( pt 1 ) :231-238.
  • 10Sui G, Soohoo C, Affarel B, et al. A DNA vector-based RNAi technology to suppress gene expression in mammalian cells [ J ].Proc Natl Acad Sci USA, 16, 2002 ,99(8) :5515-5520.

共引文献17

同被引文献1

引证文献1

国际泌尿系统杂志
2007年 第4期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部