雌激素对肝脏撞击伤后免疫功能的影响

Effects of estrogen on immunoregulation after hepatic impact injury

目的探讨雌激素对肝脏撞击伤后免疫功能的影响及可能的机制。方法将18只雄性新西兰兔随机分A组:伤前不应用雌激素;B组:伤前应用常规剂量雌二醇(1mg/kg);C组:伤前应用大剂量雌二醇(5mg/kg)。BIM-Ⅳ型生物撞击机撞击动物剑突致伤。测定外周血肿瘤坏死因子-α(TNF-α)、白介素-6(IL-6)、白介素-8(IL-8),同时检测肝脏组织中性粒细胞浸润及肝脏组织雌激素受体表达。结果实验动物肝损伤发生率100%。B组、C组与A组比较,血清TNF-α、IL-6、IL-8、肝脏组织髓过氧化物酶活性均显著低于A组(P<0.01),且B组与C组比较,C组明显低于B组(P<0.05);B组及C组血清雌二醇(E2)、肝脏组织ER表达阳性率均显著高于A组(P<0.01),且B组与C组比较,C组明显高于B组(P<0.05)。结论雌激素剂量依赖上调靶器官雌激素受体水平,从而减少促炎细胞因子生成及降低组织中性粒细胞浸润。雌激素可能成为一种新的用于雄性动物创伤后免疫功能紊乱的调节剂。

Objective To investigate the effects of estrogen on immunoregulation after hepatic impact injury, and its possible mechanism. Methods Eighteen rabbits were divided randomly into Group A simple impact group, Group B normal dosage estrogen-impact group （benzestrofol l mg/kg） , Group C high dosage estrogen-impact group （benzestrofol 5mg/kg）. Hepatic impact injury was caused by BIM-IV bio-impactor at appendix ensiformis. TNF-α, IL-6, IL-8, neutrophils infiltration （ using myleoperoxidase activity, MPO ） ,liver tissues estrogen receptors expres- sion were measured by immunohistochemistry. Results TNF-α, IL-6, IL-8,liver tissues MPO were remarkably low-er in the group B and the group C than that in the group A（P〈0.01 ）. It was lower in the group C than that in the group B（ P 〈0.05 ） ; Serum E2 and liver estrogen receptor express were higher in the group B and the group C than that in the group A （P 〈 0.01 ） ,and it was higher in the group C than that in the group B（ P 〈 0.05）. Conclusion Estrogen attenuated the immune suppression after trauma dose-dependently, and mainly mediated by elevated target organ estrogen receptor. Estrogen might be a novel immunoprotection adjunct for posttraumatic male animals.