巴楚蘑菇多糖提取工艺研究

STUDY ON POLYSACCHARIDE EXTRACTING CONDITION OF BACHU MUSHROOM

采用单因素和正交试验研究巴楚蘑菇可溶性粗多糖的提取工艺,并通过乙醇浓度对醇析保留率进行实验,确定最适醇沉终浓度。结果显示:影响多糖提取因素的主次顺序为温度>pH>料液比>时间,最佳提取工艺为提取温度95℃,料液比1∶25,时间90min,pH7,提取两次,多糖得率为8.7%,酸碱条件不利于多糖的提取;最适醇沉终浓度80%,保留率达到93.8%。

The extracting of crude polysaccharide from bachu mushroom is studied through single factorial test and orthogonal experiments. The optimal ethanol precipitation is determined. The results show the temperature is the main factor, the optimum extracting condition is temperature 95 ~C, solid-liquid ratio 1：25, extracting time 90 min, pH7. Under these conditions, the extracting percent is 8.7 %. Using 80 % ethanol for precipitating carse polysaccharide, retain ratio is 93.8 %.