摘要
目的探讨叶黄素对食管癌EC9706细胞诱导分化的影响及其机制。方法食管癌EC9706细胞采用开放式单层贴壁培养。实验设溶剂对照及叶黄素3个剂量组,采用MTT法及流式细胞术,对EC9706细胞的增殖和细胞周期进行分析,HE染色对细胞的形态学进行观察,酸解DNA甲绿-派洛宁技术了解增殖与分化型细胞比例,免疫组化检测cyclinD1的表达。结果与溶剂对照组比较,叶黄素(100μg/mL和150μg/mL)可明显抑制EC9706细胞的增殖,阻滞细胞周期于G0/G1期,降低细胞增殖指数,细胞形态趋向良性分化,cyclinD1表达水平降低。结论叶黄素可通过抑制cyclinD1表达而介导食管癌EC9706细胞增殖抑制和诱导成熟分化。
Objective The purpose of the study was to explore the effects and molecular mechanisms of lutein on the differentiation of esophagus cancer EC9706 cell. Methods EC9706 cells were seeded in 1640 medium before the addition of test compounds. The respective test compound was added in fresh medium and the control cell received the vehicle (DMSO) or Fluorouracil. The proliferation and cell cycle of EC9706 were determined by MTT assay and flow cytometry, respectively. The change in cytomorphology was investigated by using HE staining. Proliferation and differentiation cells were checked and observed by methyl green-pyronine staining. The protein expression of cyclin D1 was detected by immunohistochemistry. Results Compared with the DMSO control group, the proliferation of the EC9706 cells treated with lutein (100 μg/mL and 150 μg/mL) could markedly be decreased and the cell cycle was blocked at G0/G1 phage which caused significant changes in the cytomorphology of EC9706 cell line, and the cell malignant degree tended to drop down, the protein expression of cyclin D1 was also down-regulated significantly. Conclusion Lutein can inhibit the proliferation of EC9706 cell, and promote the cancer cell differentiation, cyclin D1 may be involved in cell proliferation and differentiation events in esophageal cancer EC9706 cell, which is regulated by lutein.
出处
《四川大学学报(医学版)》
CAS
CSCD
北大核心
2007年第4期629-632,共4页
Journal of Sichuan University(Medical Sciences)