可生物降解凝胶膜预防腹腔术后粘连的实验研究

The Experimental Study on Biodegradable Gel Membrane Applied to Prevention of Postoperative Adhesion of Abdominal Cavity Surgery

目的拟研制一种新型的防肠粘连的材料及其应用剂型,并探索该剂型材料的应用技术和效果。方法采用新西兰大白兔24只,分为实验组(18只)和对照组(6只),全麻下于兔回盲部结肠浆膜层人工形成创面,建立腹腔手术的动物模型。实验组创面用可生物降解凝胶膜对创面进行喷涂保护,对照组创面用生理盐水冲洗,缝合关闭腹腔。分别于术后2周、4周、8周处死动物取样,行大体和组织学观察。结果实验组术后2周、4周肠壁间可见有少许的粘连带,术后8周肠壁间粘连带消失不见。对照组术后2周可见肠壁间有少许粘连带,术后4周肠壁间可见明显粘连带,术后8周见条索状瘢痕。结论该生物降解凝胶膜能有效的预防术后肠粘连,并有良好的操作性。

Objective To develop a new type of anti-adhesion gel membrane and explore its applying technique. Methods 24 adult New Zealand white rabbits were used for the experiment research project, the animals were divided into two groups： the experiment group （18 adult New Zealand white rabbits） and the control group （6 adult New Zealand white rabbits）. The animal models were established via the abdominal cavity. The biodegradable gel membrane was covered to the surface of the operating region in experimental animal group, while the 0. 9%NaCL was directly rinsing the operating region in the controlled group. The specimens were collected at postoperatively week 2, 4 or 8 i＇espectively. The samples were evaluated by global and histological observation. Results In the experiment group, a few adhed zones were observed in 2 and 4 weeks after operation, but in 8 weeks after operation, the adhesion zone was disappeared. In the control group, a few adheol zones were founded in 2 weeks after operation. In 4 weeks after operation, the adhed zone become conspicuously emerged. In 8 weeks after operation, the cicatri band was occurred. Conclusion The biodegradable gel membrane usd into the postoperative abdominal cavity is effective and easy to manipulate, and it could be used as a high-effect, cheap drug of anti-adhesion in operation for surgeons.